Assembly of functionally active Drosophila origin recognition complex from recombinant proteins

被引：84

作者：

Chesnokov, I ^{[1
]}

Gossen, M ^{[1
]}

Remus, D ^{[1
]}

Botchan, M ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

GENES & DEVELOPMENT | 1999年 / 13卷 / 10期

关键词：

DNA replication; ORC; Drosophila; gene mapping;

D O I：

10.1101/gad.13.10.1289

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In eukaryotes the sites for the initiation of chromosomal DNA replication are believed to be determined in part by the binding of a heteromeric origin recognition complex (ORC) to DNA. We have cloned the genes encoding the subunits of the Drosophila ORC. Each of the genes is unique and can be mapped to discrete chromosomal locations implying that the pattern and developmental regulation of origin usage in Drosophila is not regulated solely by a large family of different ORC proteins. The six-subunit ORC can be reconstituted with recombinant proteins into a complex that restores DNA replication in ORC-depleted Drosophila or Xenopus egg extracts.

引用

页码：1289 / 1296

页数：8

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