Molecular genetic evidence of the ability of alternative oxidase to support respiratory carbon metabolism

被引:61
作者
Vanlerberghe, GC
Vanlerberghe, AE
McIntosh, L
机构
[1] MICHIGAN STATE UNIV, US DOE, PLANT RES LAB, E LANSING, MI 48824 USA
[2] MICHIGAN STATE UNIV, DEPT BIOCHEM, E LANSING, MI 48824 USA
[3] UNIV TORONTO, DIV LIFE SCI, SCARBOROUGH, ON M1C 1A4, CANADA
[4] UNIV TORONTO, DEPT BOT, SCARBOROUGH, ON M1C 1A4, CANADA
关键词
D O I
10.1104/pp.113.2.657
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
With the cytochrome pathway inhibited, AOX was able to support considerable growth of cultured tobacco (Nicotiana tabacum cv Petit Havana SR1) cells but the efficiency of carbon utilization decreased dramatically. Antisense cells with decreased AOX protein did not grow, whereas sense cells with elevated AOX protein had higher growth and respiration rates than the wild type. In antisense cells a large accumulation of pyruvate resulted in aerobic ethanolic fermentation.
引用
收藏
页码:657 / 661
页数:5
相关论文
共 23 条
[21]   ALTERNATIVE OXIDASE ACTIVITY IN TOBACCO LEAF MITOCHONDRIA - DEPENDENCE ON TRICARBOXYLIC-ACID CYCLE-MEDIATED REDOX REGULATION AND PYRUVATE ACTIVATION [J].
VANLERBERGHE, GC ;
DAY, DA ;
WISKICH, JT ;
VANLERBERGHE, AE ;
MCINTOSH, L .
PLANT PHYSIOLOGY, 1995, 109 (02) :353-361
[22]   MITOCHONDRIAL ELECTRON-TRANSPORT REGULATION OF NUCLEAR GENE-EXPRESSION - STUDIES WITH THE ALTERNATIVE OXIDASE GENE OF TOBACCO [J].
VANLERBERGHE, GC ;
MCINTOSH, L .
PLANT PHYSIOLOGY, 1994, 105 (03) :867-874
[23]  
WAGNER AM, 1992, MOLECULAR, BIOCHEMICAL AND PHYSIOLOGICAL ASPECTS OF PLANT RESPIRATION, P609