Fabrication of a microfluidic cell analyzer in a microchannel using impedance spectroscopy

被引：11

作者：

Gawad, S ^{[1
]}

Heuschkel, M ^{[1
]}

Leung-Ki, Y ^{[1
]}

Iuzzolino, R ^{[1
]}

Schild, L ^{[1
]}

Lerch, P ^{[1
]}

Renaud, P ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Swiss Fed Inst Technol, DMT, IMS, CH-1015 Lausanne, Switzerland

来源：

1ST ANNUAL INTERNATIONAL IEEE-EMBS SPECIAL TOPIC CONFERENCE ON MICROTECHNOLOGIES IN MEDICINE & BIOLOGY, PROCEEDINGS | 2000年

关键词：

D O I：

10.1109/MMB.2000.893791

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The design, fabrication techniques and characterization of an on-chip flow-cytometer device as well as the packaging approach is described. The device consists of planar microelectrodes situated on one or both sides of a micro-channel connected to an electronic detection circuitry. Simulations of the cells effect on the channel impedance are presented and compared with real measurements. Efforts have particularly been focused on realizing an interconnnecting setup to provide easy electrical and fluidic connections, to allow good monitoring and easy handling of the chip. Our cell analyzer is aimed at diagnostic applications for cell counting and separation in hematology (blood cells, leukocytes and reticulocytes). Another possible application is a micro-cell sorter that can be used to separate abnormal cell types that are present in samples at very low number such as cancer cells (cancer cytology).

引用

页码：297 / 301

页数：5

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