Activation of cellulose membranes with 1,1′-carbonyldiimidazole or 1-cyano-4-dimethylaminopyridinium tetrafluoroborate as a basis for the development of immunosensors

被引：40

作者：

Stöllner, D ^{[1
]}

Scheller, FW ^{[1
]}

Warsinke, A ^{[1
]}

机构：

[1] Univ Potsdam, Inst Biochem & Biol, D-14476 Golm, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 304卷 / 02期

关键词：

D O I：

10.1006/abio.2001.5603

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods for the activation of a cellulose dialysis membrane for immunosensor applications have been developed. For activation two reagents, 1,1'-carbonyldiimidazole (CDI) and 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP), were compared with respect to the coupling efficiency for glucose oxidase (GOx) and 1,8-diamino-2,6-dioxa-octane. The maximum level of activation was 2.4 mumol cm(-2) for CDI and 0.2 mumol cm(-2) for CDAP activation. We observed 1.5 mug cm(-2) and 0.4 x 10(-4) U cm(-2) GOx with CDI-activated membranes whereas 1.7 mug cm(-2) and 7.2 x 10(-4) U cm(-2) GOx were observed with CDAP-activated membranes. With 1,8-diamino-2,6-dioxaoctane amino group densities of 0.165 and 0.09 mumol cm(-2) were observed via CDI and CDAP activation, respectively. An amino-modified membrane was used for coupling a ligand (pentapeptide) and an immunoenzymometric assay for hemoglobin A1c was carried out. (C) 2002 Elsevier Science (USA).

引用

页码：157 / 165

页数：9

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