Fibre-optic genosensor for specific determination of femtomolar DNA oligomers

被引：51

作者：

Kleinjung, F ^{[1
]}

Bier, FF ^{[1
]}

Warsinke, A ^{[1
]}

Scheller, FW ^{[1
]}

机构：

[1] UNIV POTSDAM, INST BIOCHEM & MOL PHYSIOL, MAX DELBRUCK CTR MOL MED, D-13125 BERLIN, GERMANY

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 350卷 / 1-2期

关键词：

DNA-probe; biosensors; fiber optic fluorescence;

D O I：

10.1016/S0003-2670(97)00192-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The binding of DNA oligonucleotides to immobilized DNA-targets using a fibre optic fluorescence sensor is demonstrated. 13mer oligonucleotides were attached to the core of a multimode fibre. The complementary sequence was detected by use of a fluorescent double strand specific DNA ligand (YOYO and PicoGreen). The evanescent field was employed to distinguish between bound and not bound species. The template DNA-oligomer was immobilized either by direct coupling to the activated sensor surface or using the avidin-biotin bridge. Single base mismatches in the target sequence were detected; and a detection limit of the sensor of 30 fM (3.2 attomoles) was found for the matching target.

引用

页码：51 / 58

页数：8

共 38 条

[1] Fiber-optic evanescent wave biosensor for the detection of oligonucleotides
Abel, AP
Weller, MG
Duveneck, GL
Ehrat, M
Widmer, HM
[J]. ANALYTICAL CHEMISTRY, 1996, 68 (17) : 2905 - 2912
[2] Abramson Richard D., 1993, Current Opinion in Biotechnology, V4, P41, DOI 10.1016/0958-1669(93)90030-Z
[3] Alford R. Lynn, 1994, Current Opinion in Biotechnology, V5, P29, DOI 10.1016/S0958-1669(05)80066-7
[4] [Anonymous], METHOD ENZYMOL
[5] BIER, UNPUB
[6] Label-free observation of DNA-hybridisation and endonuclease activity on a wave guide surface using a grating coupler
Bier, FF
Scheller, FW
[J]. BIOSENSORS & BIOELECTRONICS, 1996, 11 (6-7) : 669 - 674
[7] USE OF A FIBER OPTIC IMMUNOSENSOR FOR THE DETECTION OF PESTICIDES
BIER, FF
STOCKLEIN, W
BOCHER, M
BILITEWSKI, U
SCHMID, RD
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 1992, 7 (1-3) : 509 - 512
[8] BIER FF, IN PRESS SENS ACT B
[9] BIER FF, 1996, FRONTIERS BIOSENSORI, V1, P95
[10] Direct observation of affinity reactions by reflected-mode operation of integrated optical grating coupler
Brandenburg, A
Polzius, R
Bier, F
Bilitewski, U
Wagner, E
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 1996, 30 (01) : 55 - 59

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