Fission Yeast Scm3 Mediates Stable Assembly of Cnp1/CENP-A into Centromeric Chromatin

被引：160

作者：

Williams, Jessica S. ^{[1
]}

Hayashi, Takeshi ^{[3
]}

Yanagida, Mitsuhiro ^{[3
]}

Russell, Paul ^{[1
,2
]}

机构：

[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 90237 USA

[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 90237 USA

[3] Kyoto Univ, Japan Sci & Technol Corp,Grad Sch Biostudies, CREST Res Program,Dept Gene Mech, Sakyo Ku, Kyoto 6068501, Japan

来源：

MOLECULAR CELL | 2009年 / 33卷 / 03期

关键词：

CENP-A; HISTONE CHAPERONES; PROTEIN; MIS6; DISTINCT; DOMAIN; GENE; CSE4;

D O I：

10.1016/j.molcel.2009.01.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mis16 and Mis18 are subunits of a protein complex required for incorporation of the histone H3 variant CenH3 (Cnp1/CENP-A) into centromeric chromatin in Schizosaccharomyces pombe and mammals. How the Mis16-Mis18 complex performs this function is unknown. Here, we report that the Mis16-Mis18 complex is required for centromere localization of Scm3(Sp), a Cnp1-binding protein related to Saccharomyces cerevisiae Scm3. Scm3(Sp) is required for centromeric localization of Cnp1, while Scm3(Sp) localizes at centromeres independently of Cnp1. Like the Mis16-Mis18 complex but unlike Cnp1, Scm3(Sp) dissociates from centromeres during mitosis. Inactivation of Scm3(Sp) or Mis18 increases centromere localization of histories H3 and H2A/H2B, which are largely absent from centromeres in wild-type cells. Whereas S. cerevisiae Scm3 is proposed to replace histone H2A/H2B in centromeric nucleosomes, the dynamic behavior of S. pombe Scm3 suggests that it acts as a Cnp1 assembly/maintenance factor that directly mediates the stable deposition of Cnp1 into centromeric chromatin.

引用

页码：287 / 298

页数：12

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