Enzymatic modification of alginates with the mannuronan C-5 epimerase AlgE4 enhances their solubility at low pH

被引：16

作者：

Hartmann, M

Dentini, M

Draget, KI

Skjåk-Bræk, G

机构：

[1] Univ Sci & Technol NTNU, Dept Biotechnol, Norwegian Biopolymer Lab, N-7491 Trondheim, Norway

[2] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy

来源：

CARBOHYDRATE POLYMERS | 2006年 / 63卷 / 02期

关键词：

alginate; C-5; epimerase; acid solubility;

D O I：

10.1016/j.carbpol.2005.08.052

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The recent availability of mannuronan C-5 epimerase from Azotobacter vinelandii opens up for modifying the sequential in a controlled way. To investigate the effect of an increased amount of alternating sequences on the acid solubility of alginate, different alginates from Durvillea antarctica, Lessonia nigrescens, Laminaria hyperborea and a bacterial mannuronan were epimerized using AlgE4. This enzyme is produced recombinantly in Escherichia coli and converts the M blocks into MGM sequences leaving the G-blocks intact. The solubility of the modified alginates was investigated as a function of pH and time. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：257 / 262

页数：6

共 14 条

[1] Biochemical analysis of the processive mechanism for epimerization of alginate by mannuronan C-5 epimerase AlgE4 [J].