Use of photoactivation and photobleaching to monitor the dynamic regulation of E-cadherin at the plasma membrane

被引：18

作者：

Canel, Marta ^{[1
]}

Serrels, Alan ^{[1
]}

Anderson, Kurt I. ^{[2
]}

Frame, Margaret C. ^{[1
]}

Brunton, Valerie G. ^{[1
]}

机构：

[1] Univ Edinburgh, Edinburgh Canc Res Ctr, Inst Genet & Mol Med, Edinburgh, Midlothian, Scotland

[2] Beatson Inst Canc Res, Glasgow G61 1BD, Lanark, Scotland

来源：

CELL ADHESION & MIGRATION | 2010年 / 4卷 / 04期

关键词：

E-cadherin; adherens junctions; plasma membrane; protein dynamics; photoactivation; photobleaching;

D O I：

10.4161/cam.4.4.12661

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The dynamic control of E-cadherin is critical for establishing and maintaining cell-cell junctions in epithelial cells. The concentration of E-cadherin molecules at adherens junctions (AJs) is regulated by lateral movement of E-cadherin within the plasma membrane and endocytosis. Here we set out to study the interplay between these processes and their contribution to E-cadherin dynamics. Using photoactivation (PA) and fluorescence recovery after photobleaching (FRAP), we were able to monitor the fate of E-cadherin molecules within the plasma membrane. Our results suggest that the motility of E-cadherin within and away from the cell surface are not exclusive or independent mechanisms and there is a fine balance between the two which, when perturbed, can have dramatic effects on the regulation of AJs.

引用

页码：491 / 501

页数：11

共 34 条

[21]

Nollet F, 1999, Mol Cell Biol Res Commun, V2, P77, DOI 10.1006/mcbr.1999.0155

[22] Cadherin-mediated cell-cell adhesion: sticking together as a family [J].