Novel insecticidal toxins from the venom of the spider Segestria florentina

被引：23

作者：

Lipkin, A

Kozlov, S

Nosyreva, E

Blake, A

Windass, JD ^{[1
]}

Grishin, E

机构：

[1] Russian Acad Sci, Lab Neororeceptors & Neuroregulators, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 109017, Russia

[2] Jealotts Hill Int Res Stn, Lead Characterisat Sect, Bracknell, Berks, England

来源：

TOXICON | 2002年 / 40卷 / 02期

关键词：

Segstria florentina; spider venom; insecticidal polypeptide toxins; flaccid paralysis;

D O I：

10.1016/S0041-0101(01)00181-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Three insecticidal polypeptide toxins (F5.5, F5.6, F5.7) with molecular masses 4973, 4993 and 5159 Da were isolated from the venom of the central Asian spider Segestria florentina. These toxins caused the complete flaccid paralysis of Heliothis virescens (Lepidoptera: Noctuidae) larvae (LD50 4-10 mug/g), whereas they were inactive upon intravenous injections into mice. On the basis of N-terminal amino acid sequences a family of eight genes encoding highly homologues polypeptides (SFI1-SFI8) was revealed, some of which encode polypeptides actually demonstrated to be present in S. florentina venom. All deduced polypeptides consist of 46 amino acids residues. Comparison of primary structures of SFI1-SFI8 with other spider toxins suggests that this family might share structural and functional relationships with other small spider neurotoxins, several of which are known to be highly selective agonists/antagonists of different voltage-dependent Ca2+ channels. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：125 / 130

页数：6

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