Repopulation of laser-perforated chondroepiphyseal matrix with xenogeneic chondrocytes: An experimental model

被引:11
作者
Caruso, EM [1 ]
Lewandrowski, KU [1 ]
Ohlendorf, C [1 ]
Tomford, WW [1 ]
Zaleske, DJ [1 ]
机构
[1] MASSACHUSETTS GEN HOSP, ORTHOPAED RES LABS, PEDIAT ORTHOPAED SERV, WACC 507, BOSTON, MA 02114 USA
关键词
D O I
10.1002/jor.1100140117
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Growth of chondrocytes into a xenogeneic chondroepiphyseal matrix was investigated in an in vitro experimental model by combining viable calf chondrocytes with chick epiphyseal matrix devoid of viable chondrocytes. The chondrocytes were harvested from the wrist joints of newborn calves and cultured for 2 days. The epiphyses were harvested from the distal femurs and the proximal tibias of fetal chicks after development was arrested at 17 days by freezing, The epiphyseal specimens were prepared in four ways. These included femoral and tibial epiphyses without holes and femoral and tibial epiphyses with holes made by a laser, These epiphyseal specimens were co-cultured with calf chondrocytes for various periods. After digestion of the epiphyseal matrix, viable chondrocytes were counted in suspension, Chondrocyte division in the matrix was assessed by [H-3]thymidine incorporation. The growth of calf chondrocytes into the xenogeneic chick matrix was evaluated by fluorescence microscopy on fresh thick epiphyseal sections, The percentage of viable chondrocytes in the xenogeneic epiphyseal matrix increased with culture time to a maximum al day 21. The addition of laser-drilled holes was found to extend a plateau of chondrocyte viability until day 29. A decrease in cell viability was detected at later observation points. This study demonstrates that xenogeneic matrix may serve as a morphogenetic scaffold for chondrocytic growth.
引用
收藏
页码:102 / 107
页数:6
相关论文
共 22 条
[21]   HEMIEPIPHYSEAL RECONSTRUCTION USING TISSUE DONATED FROM FETAL LIMBS IN A MURINE MODEL [J].
WOLOHAN, MJ ;
ZALESKE, DJ .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1991, 9 (02) :180-185
[22]   EPIPHYSEAL REPLACEMENT USING DEVELOPING TISSUE DONORS IN A MURINE MODEL - A COMBINED HISTOLOGIC AND RADIOGRAPHIC STUDY [J].
ZALESKE, DJ ;
FLOYD, WE ;
HALLETT, J ;
KUSHNER, D ;
JUPITER, J ;
TRAHAN, C ;
EHRLICH, MG ;
MANKIN, HJ .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1988, 6 (02) :155-165