Phosphorylation of linker histone H1 regulates gene expression in vivo by mimicking H1 removal

被引：109

作者：

Dou, YL ^{[1
]}

Mizzen, CA ^{[1
]}

Abrams, M ^{[1
]}

Allis, CD ^{[1
]}

Gorovsky, MA ^{[1
]}

机构：

[1] Univ Rochester, Dept Biol, Rochester, NY 14627 USA

来源：

MOLECULAR CELL | 1999年 / 4卷 / 04期

关键词：

D O I：

10.1016/S1097-2765(00)80215-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two Tetrahymena strains were created by gene replacement. One contained H1 with all phosphorylation sites mutated to alanine, preventing phosphorylation. The other had these sites changed to glutamic acid, mimicking the fully phosphorylated state. Global gene expression was not detectably changed in either strain. Instead, H1 phosphorylation activated or repressed specific genes in a manner that was remarkably similar to the effects of knocking out the gene encoding H1. These studies demonstrate a role for H1 phosphorylation in the regulation of transcription in vivo and suggest that it acts by mimicking the partial removal of H1.

引用

页码：641 / 647

页数：7

共 49 条

[1] Alteration of cell cycle-dependent histone phosphorylations by okadaic acid - Induction of mitosis-specific H3 phosphorylation and chromatin condensation in mammalian interphase cells [J].