Crystal structure of the 80S yeast ribosome

被引：103

作者：

Jenner, Lasse ^{[1
,2
,3
,4
]}

Melnikov, Sergey ^{[1
,2
,3
,4
]}

de Loubresse, Nicolas Garreau ^{[1
,2
,3
,4
]}

Ben-Shem, Adam ^{[1
,2
,3
,4
]}

Iskakova, Madina ^{[1
,2
,3
,4
]}

Urzhumtsev, Alexandre ^{[1
,2
,3
,4
,5
]}

Meskauskas, Arturas ^{[6
,7
]}

Dinman, Jonathan ^{[7
]}

Yusupova, Gulnara ^{[1
,2
,3
,4
]}

Yusupov, Marat ^{[1
,2
,3
,4
]}

机构：

[1] Inst Genet & Biol Mol & Cellulaire, F-67400 Illkirch Graffenstaden, France

[2] INSERM, U964, F-67400 Illkirch Graffenstaden, France

[3] CNRS, UMR7104, F-67400 Illkirch Graffenstaden, France

[4] Univ Strasbourg, F-67000 Strasbourg, France

[5] Univ Lorraine, Dept Phys, F-54506 Vandoeuvre Les Nancy, France

[6] Vilnius Univ, Dept Biotechnol & Microbiol, LT-10224 Vilnius, Lithuania

[7] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2012年 / 22卷 / 06期

基金：

美国国家卫生研究院;

关键词：

ANGSTROM RESOLUTION; TRANSFER-RNA; SACCHAROMYCES-CEREVISIAE; TRANSLATION INITIATION; EUKARYOTIC RIBOSOME; BACTERIAL RIBOSOME; PROTEIN-SYNTHESIS; MESSENGER-RNA; SUBUNIT; COMPLEX;

D O I：

10.1016/j.sbi.2012.07.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The first X-ray structure of the eukaryotic ribosome at 3.0 angstrom resolution was determined using ribosomes isolated and crystallized from the yeast Saccharomyces cerevisiae (Ben-Shem A, Garreau de Loubresse N, Melnikov S, Jenner L, Yusupova G, Yusupov M: The structure of the eukaryotic ribosome at 3.0 A resolution. Science 2011, 334:1524-1529). This accomplishment was possible due to progress in yeast ribosome biochemistry as well as recent advances in crystallographic methods developed for structure determination of prokaryotic ribosomes isolated from Thermus thermophilus and Escherichia coli. In this review we will focus on the development of isolation procedures that allowed structure determination (both cryo-EM and X-ray crystallography) to be successful for the yeast S. cerevisiae. Additionally we will introduce a new nomenclature that facilitates comparison of ribosomes from different species and kingdoms of life. Finally we will discuss the impact of the yeast 80S ribosome crystal structure on perspectives for future investigations.

引用

页码：759 / 767

页数：9

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