Purification, characterization and immunomodulatory activity of polysaccharides from stem lettuce

被引:193
作者
Nie, Chenzhipeng [1 ,2 ]
Zhu, Peilei [1 ,2 ,3 ]
Ma, Shuping [1 ,2 ]
Wang, Mingchun [1 ,2 ]
Hu, Youdong [4 ]
机构
[1] Anhui Agr Univ, Anhui Prov Engn Lab Agr Prod Proc, Hefei 230036, Peoples R China
[2] Anhui Agr Univ, Dept Food Sci & Engn, Hefei 230036, Anhui, Peoples R China
[3] Anhui Acad Agr Sci, Inst Hort, Hefei 230031, Peoples R China
[4] Xuzhou Med Coll, Affiliated Huaian Hosp, Huaian 223002, Peoples R China
基金
中国国家自然科学基金;
关键词
Polysaccharide; Stem lettuce; Purification; Characterization; Immunomodulatory; STRUCTURAL-CHARACTERIZATION; ANTIOXIDANT ACTIVITY; SULFATED POLYSACCHARIDE; IMMUNOLOGICAL ACTIVITY; STIMULATING ACTIVITY; ZIZANIA-LATIFOLIA; LEPIDIUM MEYENII; SWOLLEN CULMS; POLYMYXIN-B; IN-VITRO;
D O I
10.1016/j.carbpol.2018.02.009
中图分类号
O69 [应用化学];
学科分类号
070301 [无机化学];
摘要
Stem lettuce has a long history of cultivation in China and possesses high nutritional and medicinal value. In our previous studies, extraction optimization, characterization, and bioactivities of stem lettuce polysaccharides (SLP) were investigated. In this study, SLP were further separated into two purified polysaccharides, SLP-1 and SLP-2, by anion exchange chromatography followed by size exclusion chromatography. SLP-1, with a molecular weight of 90 KDa, was mainly composed of galacturonic acid, galactose and arabinose in a molar ratio of 17.6:41.7:33.9. SLP-2, with a molecular weight of 44 KDa, was mainly composed of mannose, galacturonic acid, galactose and arabinose in a molar ratio of 11.5:69.5:9.3:8.2. In addition, both purified polysaccharides contain sulphate radicals, have triple helical structures and can promote macrophage proliferation without cytotoxicity. SLP-2 was better able to stimulate phagocytic and nitric oxide production than SLP-1. The results suggest that polysaccharides from stem lettuce could be explored as immunomodulatory agents in the field of pharmaceuticals and functional foods.
引用
收藏
页码:236 / 242
页数:7
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