An engineered aryl azide ligase for site-specific mapping of protein-protein interactions through photo-cross-linking

被引：71

作者：

Baruah, Hemanta ^{[1
]}

Puthenveetil, SuJiet ^{[1
]}

Choi, Yoon-Aa ^{[1
]}

Shah, Samit ^{[1
]}

Ting, Alice Y. ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 37期

关键词：

ligases; photo-cross-linking; protein engineering; protein interactions; site-specific labeling;

D O I：

10.1002/anie.200802088

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Labeled and linked: The small-molecule binding site of Escherichia coli lipoic acid ligase was re-engineered to accept a fluorinated aryl azide probe in place of lipoic acid. Labeling with this mutant is highly specific for LAP fusion proteins. In cell lysate, FK506 binding protein was labeled and rapamycin-dependent photocross-linking to its interaction partner was demonstrated. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：7018 / 7021

页数：4

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