Inhibition of Salmonella typhimurium in the chicken intestinal tract by a transformed avirulent avian Escherichia coli

被引:37
作者
Wooley, RE [1 ]
Gibbs, PS [1 ]
Shotts, EB [1 ]
机构
[1] Univ Georgia, Coll Vet Med, Dept Med Microbiol & Parasitol, Athens, GA 30602 USA
关键词
intestinal tract; mutant Escherichia coli; Salmonella; broiler chickens;
D O I
10.2307/1592614
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
An avirulent, wild-type avian Escherichia coli (E. coli Av) was electrotransformed with a plasmid coding for the production of microcin 24 (pGOB18) and was designated E. roll AvGOB18. The transformant inhibited the growth of seven serotypes of Salmonella commonly associated with colonization and contamination of poultry products and seven strains of E. roll O157:H7 in the in vitro colicin/microcin assay. The transformant did not inhibit the replication of multiple isolates of Listeria monocytogenes or Campylobacter jejuni in similar assays. The transformant is nonconjugative, indicating that the plasmid would not be transmitted to other intestinal microflora in the environment. The transformant also survived in sterile tap and deionized water incubated at 25 C and 37 C in the laboratory for 30 days and was recovered from drinkers and birds in in vivo floor pen studies. In in vivo studies, E. coli AvGOB18 did not colonize the intestinal tract of broiler chicks when given as a single or multiple dose and did not reduce the Salmonella load in the broilers. But Salmonella typhimurium was reduced significantly in the intestinal tracts of broiler chickens when E, roll AvGOB18 was administered continually in the water supply.
引用
收藏
页码:245 / 250
页数:6
相关论文
共 26 条
[21]   Analysis of plasmids cloned from a virulent avian Escherichia coli and transformed into Escherichia coli DH5 alpha [J].
Wooley, RE ;
Gibbs, PS ;
Dickerson, HW ;
Brown, J ;
Nolan, LK .
AVIAN DISEASES, 1996, 40 (03) :533-539
[22]   CHARACTERISTICS OF CONJUGATIVE R-PLASMIDS FROM PATHOGENIC AVIAN ESCHERICHIA-COLI [J].
WOOLEY, RE ;
SPEARS, KR ;
BROWN, J ;
NOLAN, LK ;
DEKICH, MA .
AVIAN DISEASES, 1992, 36 (02) :348-352
[23]   PHENOTYPIC-EXPRESSION OF RECOMBINANT PLASMIDS PKT107 AND PHK11 IN AN AVIRULENT AVIAN ESCHERICHIA-COLI [J].
WOOLEY, RE ;
NOLAN, LK ;
BROWN, J ;
GIBBS, PS ;
BOUNOUS, DI .
AVIAN DISEASES, 1994, 38 (01) :127-134
[24]   ASSOCIATION OF K-1 CAPSULE, SMOOTH LIPOPOLYSACCHARIDES, TRAT GENE, AND COLICIN-V PRODUCTION WITH COMPLEMENT RESISTANCE AND VIRULENCE OF AVIAN ESCHERICHIA-COLI [J].
WOOLEY, RE ;
NOLAN, LK ;
BROWN, J ;
GIBBS, PS ;
GIDDINGS, CW ;
TURNER, KS .
AVIAN DISEASES, 1993, 37 (04) :1092-1096
[25]   EFFECT OF NORMAL INTESTINAL FLORA OF CHICKENS ON COLONIZATION BY VIRULENT COLICIN V-PRODUCING, AVIRULENT, AND MUTANT COLICIN V-PRODUCING AVIAN ESCHERICHIA-COLI [J].
WOOLEY, RE ;
BROWN, J ;
GIBBS, PS ;
NOLAN, LK ;
TURNER, KR .
AVIAN DISEASES, 1994, 38 (01) :141-145
[26]  
Zar, 2014, BIOSTAT ANAL