Dual expression of hTERT and VEGF prolongs life span and enhances angiogenic ability of aged BMSCs

被引：27

作者：

Tang, Hao ^{[1
,2
]}

Xiang, Yongsheng ^{[1
,3
]}

Jiang, Xiaodan ^{[1
]}

Ke, Yiquan ^{[1
]}

Xiao, Zongyu ^{[1
]}

Guo, Yang ^{[1
]}

Wang, Qiujing ^{[1
]}

Du, Mouxuan ^{[1
]}

Qin, Linsha ^{[1
]}

Zou, Yuxi ^{[1
]}

Cai, Yingqian ^{[1
]}

Chen, Zhenzhou ^{[1
]}

Xu, Ruxiang ^{[1
,2
]}

机构：

[1] Southern Med Univ, Zhujiang Hosp, Dept Neurosurg, Guangzhou, Guangdong, Peoples R China

[2] Mil Gen Hosp Beijing PLA, Affiliated Bayi Brain Hosp, Dept Neurosurg, Beijing, Peoples R China

[3] Jinan Univ, Affiliated Hosp 1, Dept Neurosurg, Guangzhou, Guangdong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 440卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Bone marrow stromal cell; Genetic engineering; hTRET; VEGF; MESENCHYMAL STEM-CELLS; MARROW STROMAL CELLS; REVERSE-TRANSCRIPTASE GENE; ENDOTHELIAL-LIKE CELLS; BONE-MARROW; DIFFERENTIATION CAPACITY; FUNCTIONAL RECOVERY; TELOMERASE; TRANSPLANTATION; STROKE;

D O I：

10.1016/j.bbrc.2013.09.053

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have confirmed the therapeutic effects of bone marrow stromal cells (BMSCs) transplantation on cerebral ischemia. However, the proliferative, differentiative, and homing capacity of BMSC from the elderly are significantly reduced, especially after several passages expansion in vitro. In this study, by introducing lentivirus-mediated hTERT and VEGF genes to modify human BMSCs from aged donors, we observed extended lifespan, promoted angiogenic capacity while less enhanced tumorigenicity of the genetically engineering BMSCs. These results therefore suggest that the modification of aged BMSCs by dual expression of hTERT and VEGF may be used for autologous cell replacement for ischemic cerebrovascular disease in elderly patients. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：502 / 508

页数：7

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