The 3-D structure of microsomal glutathione transferase 1 at 6 Å resolution as determined by electron crystallography of p22121 crystals

Pure solubilised microsomal glutathione transferase I (MGST1) forms well-ordered two-dimensional (2-D) crystals of two different symmetries. one orthorhombic (p22(1)2(1)) and one hexagonal (p6). both diffracting electrons to a resolution beyond 3 A. A three-dimensional (3-D) map has previously been calculated to 6 A resolution from the hexagonal crystal form. From orthorhombic crystals we have now calculated a 6 A 3-D reconstruction displaying three repeats of four rod-like densities. These are inclined relative to the normal of the membrane plane and consistent with arising from a left-handed four-helix bundle fold, The rendered volume clearly displays the same structural features as the map previously calculated from the p6 crystal type including similar lengths and substructure of the helices, but several distinguishing features do exist. The helices are more tilted in the map calculated from the orthorhombic crystals indicating conformational flexibility. Density present on the cytosolic side is consistent with the location of the active site. In addition, the current map displays the noted similarity to subunit I of cytochrome c oxidase. (C) 2002 Elsevier Science B.V. All rights reserved.