Adipose-derived stem cell therapy for intervertebral disc regeneration:: An in vitro reconstructed tissue in alginate capsules

被引:76
作者
Gaetani, Paolo [2 ]
Torre, Maria Luisa [3 ]
Klinger, Marco [4 ]
Faustini, Massimo [1 ]
Crovato, Francesca [5 ]
Bucco, Massimo [3 ]
Marazzi, Mario [5 ]
Chlapanidas, Theodora [3 ]
Levi, Daniel [2 ]
Tancioni, Flavio
Vigo, Daniele [1 ]
Rodriguez Y Baena, Riccardo [2 ]
机构
[1] Univ Milan, Dipartimento Sci & Tecnol Vet Sicurezza Alimentar, Milan, Italy
[2] Ist Clin Humanitas, Unita Operat Neurochirurg, Milan, Italy
[3] Univ Pavia, Dipartimento Chim Farmaceut, I-27100 Pavia, Italy
[4] Univ Milan, Unita Operat Chirurg Plast 2, Ist Clin Humanitas, Milan, Italy
[5] Azienda Osped Niguarda Ca Granda, Milan, Italy
关键词
D O I
10.1089/ten.tea.2007.0330
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The degenerative pathologies of the intervertebral disc have a remarkable social impact in the industrialized countries and can provide serious disabilities in the population. The current treatment consists of conservative treatments (such as symptomatic pharmacological therapies and physiokinetic therapy) and surgical treatments (intervertebral fusion, total disc replacement, nucleus pulposus (NP) replacement, or surgical exeresis). Recent advances in cell therapy foresee the possibility of regenerating the damaged disc; the autologous disc tissue can be withdrawn, in vitro regenerated, and re-implanted. The aim of this work was to verify whether autologous adipose-derived adult stem cells can improve the quality of an in vitro reconstructed nucleus pulposus tissue. A three-dimensional (3D) co-culture of NP cells and adipose tissue non-adipocyte fraction cells (nAFs) was assessed in a previously developed alginate 3D culture system following the good manufacturing practice guidelines to ensure patient safety for clinical studies. Morphological investigation of cultured and co-cultured cells was performed using transmission electron microscopy and immunofluorescence for collagen type I, aggrecan, CD90, CD34, and vimentin. Results indicate that co-culture of NP and nAFs improves the quality of the in vitro reconstructed tissue in term of extracellular matrix production and 3D cell organization. Technological resources are available for NP cell encapsulation intended for regenerating the intervertebral disc.
引用
收藏
页码:1415 / 1423
页数:9
相关论文
共 68 条
[1]
Notochordal cells interact with nucleus pulposus cells: Regulation of proteoglycan synthesis [J].
Aguiar, DJ ;
Johnson, SL ;
Oegema, TR .
EXPERIMENTAL CELL RESEARCH, 1999, 246 (01) :129-137
[2]
A biological approach to treating disc degeneration: not for today, but maybe for tomorrow [J].
Alini, M ;
Roughley, PJ ;
Antoniou, J ;
Stoll, T ;
Aebi, M .
EUROPEAN SPINE JOURNAL, 2002, 11 (Suppl 2) :S215-S220
[3]
Biological repair of intervertebral disc [J].
An, HS ;
Thonar, EJMA ;
Masuda, K .
SPINE, 2003, 28 (15) :S86-S92
[4]
Collagen gene expression and mechanical properties of intervertebral disc cell-alginate cultures [J].
Baer, AE ;
Wang, JY ;
Kraus, VB ;
Setton, LA .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2001, 19 (01) :2-10
[5]
Transient transfection of porcine granulosa cells after 3D culture in barium alginate capsules [J].
Benzoni, E ;
Torre, ML ;
Faustini, M ;
Stacchezzini, S ;
Cremonesi, F ;
Conte, U ;
Villani, S ;
Russo, V ;
Ricevuti, G ;
Vigo, D .
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 2005, 18 (04) :677-681
[6]
Cell therapy for disc degeneration - potentials and pitfalls [J].
Brisby, H ;
Tao, H ;
David, DF ;
Diwan, AD .
ORTHOPEDIC CLINICS OF NORTH AMERICA, 2004, 35 (01) :85-+
[7]
CHANG TMS, 1998, J BIOMATER ARTIF CEL, V16, P1
[8]
Mature human hepatocytes from ex vivo differentiation of alginate-encapsulated hepatoblasts [J].
Cheng, Nancy ;
Wauthier, Eliane ;
Reid, L. M. .
TISSUE ENGINEERING PART A, 2008, 14 (01) :1-7
[9]
CONTE U, 1998, Patent No. 0922451
[10]
Intervertebral disc cell therapy for regeneration: Mesenchymal stem cell implantation in rat intervertebral discs [J].
Crevensten, G ;
Walsh, AJL ;
Ananthakrishnan, D ;
Page, P ;
Wahba, GM ;
Lotz, JC ;
Berven, S .
ANNALS OF BIOMEDICAL ENGINEERING, 2004, 32 (03) :430-434