Effect of liposomes on energy-dependent uptake of the antioxidant SkQR1 by isolated mitochondria

被引：9

作者：

Antonenko, Yuri N. ^{[1
,3
]}

Perevoshchikova, Irina V. ^{[1
]}

Rokitskaya, Tatyana I. ^{[1
]}

Simonyan, Ruben A. ^{[1
]}

Tashlitsky, Vadim V. ^{[2
]}

Skulachev, Vladimir P. ^{[1
,3
]}

机构：

[1] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Moscow 119991, Russia

[2] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119991, Russia

[3] Moscow MV Lomonosov State Univ, Inst Mitoengn, Moscow 119991, Russia

来源：

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES | 2012年 / 44卷 / 04期

基金：

俄罗斯基础研究基金会;

关键词：

Mitochondria; Liposomes; Hydrophobic cations; Antioxidants; Bilayer lipid membranes; TARGETED PLASTOQUINONE DERIVATIVES; INTERRUPT EXECUTION; RAT-LIVER; MEMBRANE; BINDING; CELLS; SENESCENCE; CATIONS; TOOLS; IONS;

D O I：

10.1007/s10863-012-9449-9

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

The mitochondria-targeted antioxidant SkQR1 composed of a plastoquinone part covalently bound to a cationic rhodamine 19 moiety via a decane linker was previously shown to effectively protect brain and kidney from ischemia injury accompanying generation of reactive oxygen species. In the present paper the energy-dependent SkQR1 uptake by isolated rat liver mitochondria was studied by fluorescence correlation spectroscopy peak intensity analysis (FCS PIA). This approach can be used to measure the number of fluorescent molecules per single mitochondrion. A large portion of SkQR1 appeared to be taken up by mitochondria in an energy-independent fashion because of its high affinity to membranes. Liposomes were found to compete effectively with mitochondria for the energy-independent SkQR1 binding, thereby facilitating, as an "SkQR1-buffer", observation of energy-dependent SkQR1 accumulation in mitochondria. The rate of energy-dependent SkQR1 uptake by mitochondria observed in the presence of liposomes was rather low (minutes) which was apparently due to slow redistribution of SkQR1 between liposomal and mitochondrial membranes. This can explain the low rate of staining of mitochondria by SkQR1 in living cells containing, besides mitochondria, other membrane components (endoplasmic reticulum, Golgi membranes, endosomes, lysosomes, etc.) which can compete with mitochondria for the energy-independent SkQR1 binding.

引用

页码：453 / 460

页数：8

共 34 条

[1]

Alberts B., 2002, The shape and structure of proteins, Vfourth, DOI 10.1093/aob/mcg023

[2]

COUPLING MEMBRANES AS ENERGY-TRANSMITTING CABLES .1. FILAMENTOUS MITOCHONDRIA IN FIBROBLASTS AND MITOCHONDRIAL CLUSTERS IN CARDIOMYOCYTES [J].

AMCHENKOVA, AA ;

BAKEEVA, LE ;

CHENTSOV, YS ;

SKULACHEV, VP ;

ZOROV, DB .

JOURNAL OF CELL BIOLOGY, 1988, 107 (02) :481-495

[3]

[Anonymous], PHARMACIA

[4]

Mitochondria-targeted plastoquinone derivatives as tools to interrupt execution of the aging program. 1. Cationic plastoquinone derivatives: Synthesis and in vitro studies [J].

Antonenko, Y. N. ;

Avetisyan, A. V. ;

Bakeeva, L. E. ;

Chernyak, B. V. ;

Chertkov, V. A. ;

Domnina, L. V. ;

Ivanova, O. Yu. ;

Izyumov, D. S. ;

Khailova, L. S. ;

Klishin, S. S. ;

Korshunova, G. A. ;

Lyamzaev, K. G. ;

Muntyan, M. S. ;

Nepryakhina, O. K. ;

Pashkovskaya, A. A. ;

Pletjushkina, O. Yu. ;

Pustovidko, A. V. ;

Roginsky, V. A. ;

Rokitskaya, T. I. ;

Ruuge, E. K. ;

Saprunova, V. B. ;

Severina, I. I. ;

Simonyan, R. A. ;

Skulachev, I. V. ;

Skulachev, M. V. ;

Sumbatyan, N. V. ;

Sviryaeva, I. V. ;

Tashlitsky, V. N. ;

Vassiliev, J. M. ;

Vyssokikh, M. Yu. ;

Yaguzhinsky, L. S. ;

Zamyatnin, A. A., Jr. ;

Skulachev, V. P. .

BIOCHEMISTRY-MOSCOW, 2008, 73 (12) :1273-1287

[5]

Derivatives of Rhodamine 19 as Mild Mitochondria-targeted Cationic Uncouplers [J].

Antonenko, Yuri N. ;

Avetisyan, Armine V. ;

Cherepanov, Dmitry A. ;

Knorre, Dmitry A. ;

Korshunova, Galina A. ;

Markova, Olga V. ;

Ojovan, Silvia M. ;

Perevoshchikova, Irina V. ;

Pustovidko, Antonina V. ;

Rokitskaya, Tatyana I. ;

Severina, Inna I. ;

Simonyan, Ruben A. ;

Smirnova, Ekaterina A. ;

Sobko, Alexander A. ;

Sumbatyan, Natalia V. ;

Severin, Fedor F. ;

Skulachev, Vladimir P. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (20) :17831-17840

[6]

Fine-tuning the hydrophobicity of a mitochondria-targeted antioxidant [J].

Asin-Cayuela, J ;

Manas, ARB ;

James, AM ;

Smith, RAJ ;

Murphy, MP .

FEBS LETTERS, 2004, 571 (1-3) :9-16

[7]

Mitochondria-targeted plastoquinone derivatives as tools to interrupt execution of the aging program. 2. Treatment of some ROS- and Age-related diseases (heart arrhythmia, heart infarctions, kidney ischemia, and stroke) [J].

Bakeeva, L. E. ;

Barskov, I. V. ;

Egorov, M. V. ;

Isaev, N. K. ;

Kapelko, V. I. ;

Kazachenko, A. V. ;

Kirpatovsky, V. I. ;

Kozlovsky, S. V. ;

Lakomkin, V. L. ;

Levina, S. B. ;

Pisarenko, O. I. ;

Plotnikov, E. Y. ;

Saprunova, V. B. ;

Serebryakova, L. I. ;

Skulachev, M. V. ;

Stelmashook, E. V. ;

Studneva, I. M. ;

Tskitishvili, O. V. ;

Vasilyeva, A. K. ;

Victorov, I. V. ;

Zorov, D. B. ;

Skulachev, V. P. .

BIOCHEMISTRY-MOSCOW, 2008, 73 (12) :1288-1299

[8]

CHEN LB, 1988, ANNU REV CELL BIOL, V4, P155, DOI 10.1146/annurev.cellbio.4.1.155

[9]

Cunha FM, 2011, CURR DRUG TARGETS, V12, P783

[10]

DETERMINATION OF MEMBRANE-POTENTIAL WITH LIPOPHILIC CATIONS - CORRECTION OF PROBE BINDING [J].

DEMURA, M ;

KAMO, N ;

KOBATAKE, Y .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 820 (02) :207-215

← 1 2 3 4 →