Expression and secretion of Bifidobacterium adolescentis amylase by Bifidobacterium longum

被引：29

作者：

Rhim, SL

Park, MS

Ji, GE ^{[1
]}

机构：

[1] BIFIDO Co Ltd, Res Ctr, Seoul 151818, South Korea

[2] Hallym Univ, Dept Genet Engn, Chunchon 200702, South Korea

[3] Seoul Natl Univ, Dept Food Sci & Nutr, Seoul 151742, South Korea

[4] Anyang Tech Coll, Dept Nutr & Hotel Culinary Art, Gyeonggi 430749, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 03期

关键词：

amylase; Bifidobacterium; expression; secretion;

D O I：

10.1007/s10529-005-5330-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bifidobacterium adolescentis Int-57 (INT57), isolated from human feces, secretes an amylase. We have shot-gun cloned, sequence analyzed and expressed the gene encoding this amylase in B. longum. The sequenced 2477 bp fragment was homologous to other extracellular amylases. The encoded protein was predicted to be composed of 595 amino acids with a molecular weight of 64 kDa, and was designated AmyB. Highly conserved amylase domains were found in AmyB. The signal sequence and cleavage site was predicted by sequence analysis. AmyB was subcloned into pBES2, a novel E. coli-Bifidobacterium shuttle vector, to construct pYBamy59. Subsequently, B. longum, with no apparent amylase activity, was transformed with pYBamy59. More than 90% of the amylase activity was detected in the culture broth. This approach may open the way for the development of more efficient expression and secretion systems for Bifidobacterium.

引用

页码：163 / 168

页数：6

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