Development of a virus concentration method and its application to detection of enterovirus and Norwalk virus from coastal seawater

被引：403

作者：

Katayama, H ^{[1
]}

Shimasaki, A ^{[1
]}

Ohgaki, S ^{[1
]}

机构：

[1] Univ Tokyo, Dept Urban Engn, Sch Engn, Bunkyo Ku, Tokyo 1138656, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2002年 / 68卷 / 03期

关键词：

D O I：

10.1128/AEM.68.3.1033-1039.2002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed a new procedure for concentration of enteric viruses from water using a negatively charged membrane. Rinsing the membrane with 0.5 mM H2SO4 (pH 3.0) in order to elute cations prior to viral elution with 1 mM NaOH (pH 10.5) promoted poliovirus recovery yields from 33 to 95% when applied to pure water and 38 to 89% when applied to natural seawater from Tokyo Bay, Japan, respectively. This method showed average recovery yields of spiked poliovirus of 62% (n = 8) from 1 liter of artificial seawater. This method showed higher recovery yields (>61%) than that of the conventional method using positively charged membrane (6%) when applied to seawater. This method is also free from beef extract elution, which has an inhibitory effect in the subsequent viral genome detection by reverse transcription-PCR. Naturally occurring Norwalk viruses from 2 liters of Tokyo Bay water in winter and infectious enteroviruses from 2 liters of recreational coastal seawater in summer were detected by using this viral concentration method.

引用

页码：1033 / 1039

页数：7

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