Bioreactor production of camptothecin by hairy root cultures of Ophiorrhiza pumila

被引：63

作者：

Sudo, H

Yamakawa, T

Yamazaki, M

Aimi, N

Saito, K

机构：

[1] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Mol Biol & Biotechnol, Inage Ku, Chiba 2638522, Japan

[2] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Mol Struct & Biol Funct, Inage Ku, Chiba 2638522, Japan

[3] Univ Tokyo, Dept Global Agr Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 05期

基金：

日本科学技术振兴机构;

关键词：

bioreactor; camptothecin; excretion; hairy root; Ophiorrhiza pumila;

D O I：

10.1023/A:1014568904957

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A large-scale culture of hairy root of Ophiorrhiza pumila using a modified 3 l bioreactor was established. The hairy roots, incited by infection of Agrobacterium rhizogenes were grown in the bioreactor equipped with a stainless net. The final concentration of camptothecin was 0.0085% fresh wt of tissue, and the total production of camptothecin, an anti-neoplastic quinoline alkaloid, reached 22 mg over 8 weeks' culture in the reactor. Approx. 17% (3.6 mg) of the total camptothecin produced was excreted into the culture medium.

引用

收藏

页码：359 / 363

页数：5

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