Brain death does not affect hepatic allograft function and survival after orthotopic transplantation in a canine model

被引:14
作者
Compagnon, P [1 ]
Wang, H [1 ]
Lindell, SL [1 ]
Ametani, MS [1 ]
Mangino, MJ [1 ]
D'Alessandro, AM [1 ]
Southard, JH [1 ]
机构
[1] Univ Wisconsin, Dept Surg, Div Transplantat, Madison, WI 53792 USA
关键词
D O I
10.1097/00007890-200204270-00006
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Brain death has been shown to decrease graft function and survival in rodent models. The aim of this study was to evaluate how brain death affects graft viability in the donor and liver tolerance to cold preservation as assessed by survival in a canine transplant model. Methods. Beagle dogs were used for the study. Non-brain dead (BD) donors served as controls. Brain death was induced by sudden inflation of a subdural balloon catheter with continuous monitoring of arterial blood pressure and electroencephalographic activity. Sixteen hours after confirmation of brain death, liver grafts were retrieved. All livers were flushed in situ and preserved for 24 hr in cold University of Wisconsin solution before transplantation. Recipient survival rates, serum hepatic enzyme levels, coagulation, and metabolic parameters of the recipients were analyzed. Results. No significant changes were observed in serum aminotransferases (alanine and aspartate transaminases) and lactate dehydrogenase levels in the BD donor. After preservation, control (n=6) and BD livers (n=5) showed full functional recovery after transplant with 100% survival in both groups at day 7. There was no significant difference in peak serum alanine, aspartate transaminases, and lactate dehydrogenase after transplantation in recipients who received a liver from BD donor compared to control group. BD livers were functionally as capable as control livers in correcting metabolic acidosis during the first 24 hr posttransplantation. Coagulation profiles (index normalized ratio, activated partial thromboplastin time) after reperfusion were similar between groups. Conclusion. In contrast to previous reports in rodent models, our study shows that brain death does not cause significant liver dysfunction in the donor before organ removal. Donor brain death and prolonged liver graft preservation do not interact significantly to impair liver function and survival after transplantation.
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页码:1218 / 1227
页数:10
相关论文
共 72 条
[21]  
Detre K M, 1995, Liver Transpl Surg, V1, P311, DOI 10.1002/lt.500010507
[22]   ACUTE ENDOCRINE FAILURE AFTER BRAIN-DEATH [J].
GRAMM, HJ ;
MEINHOLD, H ;
BICKEL, U ;
ZIMMERMANN, J ;
VONHAMMERSTEIN, B ;
KELLER, F ;
DENNHARDT, R ;
VOIGT, K .
TRANSPLANTATION, 1992, 54 (05) :851-857
[23]   Administration of desmopressin in brain-dead donors and renal function in kidney recipients [J].
Guesde, R ;
Barrou, B ;
Leblanc, I ;
Ourahma, S ;
Goarin, JP ;
Coriat, P ;
Riou, B .
LANCET, 1998, 352 (9135) :1178-1181
[24]   Changes in organ perfusion after brain death in the rat and its relation to circulating catecholamines [J].
Herijgers, P ;
Leunens, V ;
TjandraMaga, TB ;
Mubagwa, K ;
Flameng, W .
TRANSPLANTATION, 1996, 62 (03) :330-335
[25]   HORMONAL PROFILES IN A CANINE MODEL OF THE BRAIN-DEAD ORGAN DONOR [J].
HUBER, TS ;
NACHREINER, R ;
DALECY, LG .
JOURNAL OF CRITICAL CARE, 1994, 9 (01) :7-17
[26]  
IWAI A, 1989, TRANSPLANTATION, V48, P613
[27]   Vasopressin presser effects in critically ill children during evaluation for brain death and organ recovery [J].
Katz, K ;
Lawler, J ;
Wax, J ;
O'Connor, R ;
Nadkarni, V .
RESUSCITATION, 2000, 47 (01) :33-40
[28]   Cadaver versus living donor kidneys: Impact of donor factors on antigen induction before transplantation [J].
Koo, DDH ;
Welsh, KI ;
McLaren, AJ ;
Roake, JA ;
Morris, PJ ;
Fuggle, SV .
KIDNEY INTERNATIONAL, 1999, 56 (04) :1551-1559
[29]  
KRAMER W., 1967, BRAIN RES, V6, P686, DOI 10.1016/0006-8993(67)90126-6
[30]   Activation of inflammatory mediators in rat renal isografts by donor brain death [J].
Kusaka, M ;
Pratschke, J ;
Wilhelm, MJ ;
Ziai, F ;
Zandi-Nejad, K ;
Mackenzie, HS ;
Hancock, WW ;
Tilney, NL .
TRANSPLANTATION, 2000, 69 (03) :405-410