Characterization of the human STAT5A and STAT5B promoters:: evidence of a positive and negative mechanism of transcriptional regulation

被引：29

作者：

Crispi, S ^{[1
]}

Sanzari, E ^{[1
]}

Monfregola, J ^{[1
]}

De Felice, N ^{[1
]}

Fimiani, G ^{[1
]}

Ambrosio, R ^{[1
]}

D'Urso, M ^{[1
]}

Ursini, MV ^{[1
]}

机构：

[1] Inst Genet & Biophys Adriano Buzzati Traverso, CNR, I-80131 Naples, Italy

来源：

FEBS LETTERS | 2004年 / 562卷 / 1-3期

关键词：

STAT5A; STAT5B; epigenetic control; transcriptional regulation; cis-acting element; cross-species comparison;

D O I：

10.1016/S0014-5793(04)00166-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently published the genomic characterization of the STAT5A and STAT5B paralogous genes that are located head to head in the 17q21 chromosome and share large regions of sequence identity. We here demonstrate by transient in vitro transfection that STAT5A and STAT5B promoters are able to direct comparable levels of transcription. The expression of basal promoters is enhanced after Sp1 up-regulation in HeLa and SL2 cells while DNA methylation associated to the recruitment of MeCP2 methyl CpG binding protein down-regulates STAT5A and B promoters by interfering with Sp1-induced transcription. In addition, cross-species sequence comparison identified a bi-directional negative cis-acting regulatory element located in the STAT5 intergenic region. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：27 / 34

页数：8

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