A molecular determinant for the establishment of sister chromatid cohesion

被引：364

作者：

Unal, Elcin ^{[1
,2
,3
]}

Heidinger-Pauli, Jill M. ^{[1
,2
,3
]}

Kim, Woong ^{[4
]}

Guacci, Vincent ^{[1
,2
]}

Onn, Itay ^{[1
,2
]}

Gygi, Steven P. ^{[4
]}

Koshland, Douglas E. ^{[1
,2
]}

机构：

[1] Carnegie Inst, Howard Hughes Med Inst, Baltimore, MD 21218 USA

[2] Carnegie Inst, Dept Embryol, Baltimore, MD 21218 USA

[3] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

[4] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

来源：

SCIENCE | 2008年 / 321卷 / 5888期

关键词：

D O I：

10.1126/science.1157880

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chromosome segregation, transcriptional regulation, and repair of DNA double- strand breaks require the cohesin protein complex. Cohesin holds the replicated chromosomes ( sister chromatids) together to mediate sister chromatid cohesion. The mechanism of how cohesion is established is unknown. We found that in budding yeast, the head domain of the Smc3p subunit of cohesin is acetylated by the Eco1p acetyltransferase at two evolutionarily conserved residues, promoting the chromatin- bound cohesin to tether sister chromatids. Smc3p acetylation is induced in S phase after the chromatin loading of cohesin and is suppressed in G(1) and G(2)/M. Smc3 head acetylation and its cell cycle regulation provide important insights into the biology and mechanism of cohesion establishment.

引用

页码：566 / 569

页数：4

共 30 条

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