Guiding the Immune Response against Influenza Virus Hemagglutinin toward the Conserved Stalk Domain by Hyperglycosylation of the Globular Head Domain

被引：159

作者：

Eggink, Dirk ^{[1
]}

Goff, Peter H. ^{[1
,2
]}

Palese, Peter ^{[1
,3
]}

机构：

[1] Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA

[2] Icahn Sch Med Mt Sinai, Grad Sch Biomed Sci, New York, NY USA

[3] Icahn Sch Med Mt Sinai, Dept Med, New York, NY USA

来源：

JOURNAL OF VIROLOGY | 2014年 / 88卷 / 01期

关键词：

MONOCLONAL-ANTIBODIES; GLYCOSYLATION; SITES; RECOGNITION; INDUCTION; EVOLUTION; INFECTION; SELECTION; EFFICACY; EPITOPE;

D O I：

10.1128/JVI.02608-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Influenza virus hemagglutinin consists of a highly variable and immunodominant head domain and a more conserved but immunosubdominant stalk domain. We introduced seven N-linked glycosylation sites in the hemagglutinin head domain to shield the immunodominant antigenic sites. The hyperglycosylated hemagglutinin enhanced stalk-directed seroreactivity while dampening the head response in immunized mice. Upon influenza virus challenge, mice vaccinated with the hyperglycosylated hemagglutinin were better protected against morbidity and mortality than mice receiving the wild-type hemagglutinin.

引用

页码：699 / 704

页数：6

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