Self-assembled peptide-based hydrogels as scaffolds for anchorage-dependent cells

被引:578
作者
Zhou, Mi [1 ]
Smith, Andrew M. [1 ,2 ]
Das, Apurba K. [1 ,2 ,4 ]
Hodson, Nigel W. [3 ]
Collins, Richard F. [2 ]
Ulijn, Rein V. [1 ,2 ,4 ]
Gough, Julie E. [1 ]
机构
[1] Univ Manchester, Sch Mat, Ctr Mat Sci, Manchester M1 7HS, Lancs, England
[2] Univ Manchester, Manchester Interdisciplinary Bioctr, Manchester M1 7ND, Lancs, England
[3] Univ Manchester, Manchester M13 9PT, Lancs, England
[4] Univ Strathclyde, Dept Pure & Appl Chem, WestCHEM, Glasgow G1 1XL, Lanark, Scotland
基金
英国工程与自然科学研究理事会;
关键词
Minimalist design; Self-assembly; Biomimetic materials; RGD ligands; 3D culture; Anchorage-dependent cells; BIOMATERIALS; ADHESION; FIBRONECTIN; NANOFIBERS; MATRICES;
D O I
10.1016/j.biomaterials.2009.01.010
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We report here the design of a biomimetic nanofibrous hydrogel as a 3D-scaffold for anchorage-dependent cells. The peptide-based bioactive hydrogel is formed through molecular self-assembly and the building blocks are a mixture of two aromatic short peptide derivatives: Fmoc-FF (Fluorenylmethoxycarbonyl-diphenylalanine) and Fmoc-RGD (arginine-glycine-aspartate) as the simplest self-assembling moieties reported so far for the construction of small-molecule-based bioactive hydrogels. This hydrogel provides a highly hydrated, stiff and nanofibrous hydrogel network that uniquely presents bioactive ligands at the fibre surface; therefore it mimics certain essential features of the extracellular matrix. The RGD sequence as part of the Fmoc-RGD building block plays a dual role of a structural component and a biological ligand. Spectroscopic and imaging analysis using CD, FTIR, fluorescence, TEM and AFM confirmed that FF and RGD peptide sequences self-assemble into beta-sheets interlocked by pi-pi stacking of the Fmoc groups. Ibis generates the cylindrical nanofibres interwoven within the hydrogel with the presence of RGDs in tunable densities on the fibre surfaces. This rapid gelling material was observed to promote adhesion of encapsulated dermal fibroblasts through specific RGD-integrin binding, with subsequent cell spreading and proliferation; therefore it may offer an economical model scaffold to 3D-culture other anchorage-dependent cells for in-vitro tissue regeneration. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2523 / 2530
页数:8
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