βγ dimers derived from Go and Gi proteins contribute different components of adrenergic inhibition of Ca2+ channels in rat sympathetic neurones

被引:59
作者
Delmas, P
Abogadie, FC
Milligan, G
Buckley, NJ
Brown, DA
机构
[1] UCL, Dept Pharmacol, Wellcome Lab Mol Pharmacol, London WC1E 6BT, England
[2] Univ Glasgow, Inst Biomed & Life Sci, Mol Pharmacol Grp, Div Biochem & Pharmacol, Glasgow G12 8QQ, Lanark, Scotland
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1999年 / 518卷 / 01期
基金
英国惠康基金;
关键词
D O I
10.1111/j.1469-7793.1999.0023r.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. Using perforated-patch recordings, we have examined the part played by endogenous G-protein subunits: in the alpha(2)-adrenoceptor-mediated inhibition of N-type Ca2+ currents in sympathetic neurones. 2. Two components of I-Ca inhibition by noradrenaline were recorded: a prominent, high affinity and voltage-dependent pertussis toxin (PTX)-sensitive pathway and a minor, low affinity and mostly voltage-insensitive PTS-resistant pathway. 3. PTS-sensitive inhibition was reduced by microinjection of antibodies against either G alpha(oA,B) or G alpha(i1,2). The voltage-dependent fraction of inhibition was reduced by anti-G alpha(o) but not by anti-G alpha(1) antibody. 4. Antisense depletion of G alpha(oA) led to a marked reduction of noradrenaline-induced inhibition and voltage dependence. By contrast, G alpha(i) depletion attenuated noradrenergic modulation without affecting the voltage dependence. 5. Expression of the beta gamma-binding agents beta-adrenergic receptor kinase 1 (C-terminus, beta ARK1(C-ter)) or G alpha(i1) with a Cys3 to Ser mutation partially prevented noradrenergic inhibition while alpha-transducin abolished it. Residual inhibition was mostly voltage independent in cells expressing beta ARKI(C-ter) but was strongly reversed by depolarization in G alpha(i1) Cys3Ser-expressing cells. 6. Expression of the PTX-resistant Ga-i1 Cys351Ile mutant in cells treated with PTX restored alpha(2)-adrenoceptor inhibition. This restored inhibition was weakly reversed by depolarization. Both the degree and voltage dependence of inhibition were correlated with the level of expression of the G alpha(il) Cys351Ile subunit. 7. Our findings identify beta gamma dimers associated with G alpha(oA) and G alpha(i) as mediators of the PTX-sensitive alpha(2)-adrenoceptor-mediated inhibition of S-type Ca2+ channels. Different beta gamma combinations may account for the differential voltage-dependent effects of G(o) and G(i) on I-Ca.
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页码:23 / 36
页数:14
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