Pyridoxine deficiency affects biomechanical properties of chick tibial bone

The mechanical integrity of bone is dependent on the bone matrix, which is believed to account for the plastic deformation of the tissue, and the mineral, which is believed to account for the elastic deformation. The validity of this model is shown in this study based on analysis of the bones of vitamin B-6-deficient and vitamin B-6-replete chick bones. In this model, when B-6-deficient and control animals are compared, vitamin B-6 deficiency has no effect on the mineral content or composition of cortical bone as measured by ash weight (63 +/- 6 vs, 58 +/- 3); mineral to matrix ratio of the FTIR spectra (4.2 +/- 0.6 vs, 4.5 +/- 0.2), line-broadening analyses of the X-ray diffraction 002 peak (beta(002) = 0.50 +/- 0.1 vs. 0.49 +/- 0.01), or other features of the infrared spectra, In contrast, collagen was significantly more extractable from vitamin B-6-deficient chick bones (20 +/- 2% of total hydroxyproline extracted vs. 10 +/- 3% p less than or equal to 0.001). The B-6-deficient bones also contained an increased amount of the reducible cross-links DHLNL, dehydro-dihydroxylysinonorleucine, (1.03 +/- 0.07 vs. 0.84 +/- 0.13 p less than or equal to 0.001); and a nonsignificant increase in HLNL, dehydro-hydroxylysinonorleucine, (0.51 +/- 0.03 vs, 0.43 +/- 0.03, p less than or equal to 0.10). There were no significant changes in bone length, bone diameter, or area moment of inertia, In four-point bending, no significant changes in elastic modulus, stiffness, offset yield deflection, or fracture deflection were detected, However, fracture load in the B-6-deficient animals was decreased from 203 +/- 35 MPa to 151 +/- 23 MPa, p less than or equal to 0.01, and offset yield load was decreased from 165 +/- 9 MPa to 125 +/- 14 MPa, p less than or equal to 0.05. Since earlier histomorphometric studies had demonstrated that the B-6-deficient bones were osteopenic, these data suggest that although proper cortical bone mineralization occurred, the alterations of the collagen resulted in changes to bone mechanical performance.