Genome Analysis and Characterisation of the Exopolysaccharide Produced by Bifidobacterium longum subsp longum 35624™

被引：73

作者：

Altmann, Friedrich ^{[1
]}

Kosma, Paul ^{[1
]}

O'Callaghan, Amy ^{[2
,3
]}

Leahy, Sinead ^{[2
,3
]}

Bottacini, Francesca ^{[2
,3
]}

Molloy, Evelyn ^{[2
,3
]}

Plattner, Stephan ^{[4
]}

Schiavi, Elisa ^{[5
,6
]}

Gleinser, Marita ^{[2
,3
]}

Groeger, David ^{[6
]}

Grant, Ray ^{[6
]}

Perez, Noelia Rodriguez ^{[5
]}

Healy, Selena ^{[4
]}

Svehla, Elisabeth ^{[1
]}

Windwarder, Markus ^{[1
]}

Hofinger, Andreas ^{[1
]}

Motherway, Mary O'Connell ^{[2
,3
]}

Akdis, Cezmi A. ^{[5
]}

Xu, Jun ^{[7
]}

Roper, Jennifer ^{[4
]}

van Sinderen, Douwe ^{[2
,3
]}

O'Mahony, Liam ^{[5
]}

机构：

[1] Univ Nat Resources & Life Sci, Vienna, Austria

[2] Univ Coll Cork, APC Microbiome Inst, Cork, Ireland

[3] Univ Coll Cork, Sch Microbiol, Cork, Ireland

[4] Alimentary Hlth, Cork, Ireland

[5] Univ Zurich, Swiss Inst Allergy & Asthma Res SIAF, Davos, Switzerland

[6] Alimentary Hlth Pharma Davos, Davos, Switzerland

[7] Procter & Gamble, Cincinnati, OH USA

来源：

PLOS ONE | 2016年 / 11卷 / 09期

基金：

爱尔兰科学基金会; 瑞士国家科学基金会;

关键词：

O-SPECIFIC POLYSACCHARIDE; PERFORMANCE LIQUID-CHROMATOGRAPHY; ASSISTED STRUCTURAL-ANALYSIS; PROBIOTIC STRAINS; GUT MICROBIOTA; CELL-WALL; HOST; LIPOPOLYSACCHARIDE; SEPARATION; MONOSACCHARIDES;

D O I：

10.1371/journal.pone.0162983

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Bifibobacterium longum subsp. longum 35624 (TM) strain (formerly named Bifidobacterium longum subsp. infantis) is a well described probiotic with clinical efficacy in Irritable Bowel Syndrome clinical trials and induces immunoregulatory effects in mice and in humans. This paper presents (a) the genome sequence of the organism allowing the assignment to its correct subspeciation longum; (b) a comparative genome assessment with other B. longum strains and (c) the molecular structure of the 35624 exopolysaccharide (EPS624). Comparative genome analysis of the 35624 strain with other B. longum strains determined that the sub-speciation of the strain is longum and revealed the presence of a 35624-specific gene cluster, predicted to encode the biosynthetic machinery for EPS624. Following isolation and acid treatment of the EPS, its chemical structure was determined using gas and liquid chromatography for sugar constituent and linkage analysis, electrospray and matrix assisted laser desorption ionization mass spectrometry for sequencing and NMR. The EPS consists of a branched hexasaccharide repeating unit containing two galactose and two glucose moieties, galacturonic acid and the unusual sugar 6-deoxy- L-talose. These data demonstrate that the B. longum 35624 strain has specific genetic features, one of which leads to the generation of a characteristic exopolysaccharide.

引用

页数：23

共 77 条

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