Streptococcus thermophilus is able to produce a β-galactosidase active during its transit in the digestive tract of germ-free mice

被引：43

作者：

Drouault, S

Anba, J

Corthier, G ^{[1
]}

机构：

[1] INRA, Unite Ecol & Physiol Syst Digest, F-78352 Jouy En Josas, France

[2] Arilait Rech, F-75009 Paris, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2002年 / 68卷 / 02期

关键词：

D O I：

10.1128/AEM.68.2.938-941.2002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This work presents data on the application of a bacterial luciferase used to monitor gene expression of Streptococcus thermophilus in the digestive tract. The main result is that the bacterium was able to produce an active beta-galactosidase in the digestive tract, although it did not multiply during its transit. This production was enhanced when lactose (the inducer) was added to the diet.

引用

收藏

页码：938 / 941

页数：4

相关论文

共 18 条

[1]

BAYLESS TM, 1971, PEDIATRICS, V47, P1029

[2]

Corthier G, 1998, APPL ENVIRON MICROB, V64, P2721

[3]

Drouault S, 1999, APPL ENVIRON MICROB, V65, P4881

[4]

DUCLUZEA.R, 1970, ZENTBL BAKTERIOLOG P, V213, P533

[5] CLONING, ORGANIZATION, AND EXPRESSION OF THE BIOLUMINESCENCE GENES OF XENORHABDUS-LUMINESCENS [J].

FRACKMAN, S ;

ANHALT, M ;

NEALSON, KH .

JOURNAL OF BACTERIOLOGY, 1990, 172 (10) :5767-5773

[6] Phosphorylation state of HPr determines the level of expression and the extent of phosphorylation of the lactose transport protein of Streptococcus thermophilus [J].

Gunnewijk, MGW ;

Poolman, B .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (44) :34073-34079

[7] HPr(His∼P)-mediated phosphorylation differently affects counterflow and proton motive force-driven uptake via the lactose transport protein of Streptococcus thermophilus [J].

Gunnewijk, MGW ;

Poolman, B .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (44) :34080-34085

[8] THE NUCLEOTIDE-SEQUENCE OF THE LUXA AND LUXB GENES OF XENORHABDUS-LUMINESCENS HM AND A COMPARISON OF THE AMINO-ACID-SEQUENCES OF LUCIFERASES FROM 4 SPECIES OF BIOLUMINESCENT BACTERIA [J].

JOHNSTON, TC ;

RUCKER, EB ;

COCHRUM, L ;

HRUSKA, KS ;

VANDEGRIFT, V .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 170 (02) :407-415

[9] YOGURT AND FERMENTED-THEN-PASTEURIZED MILK - EFFECTS OF SHORT-TERM AND LONG-TERM INGESTION ON LACTOSE ABSORPTION AND MUCOSAL LACTASE ACTIVITY IN LACTASE-DEFICIENT SUBJECTS [J].

LEREBOURS, E ;

NDAM, CN ;

LAVOINE, A ;

HELLOT, MF ;

ANTOINE, JM ;

COLIN, R .

AMERICAN JOURNAL OF CLINICAL NUTRITION, 1989, 49 (05) :823-827

[10] EFFECT OF THE MICROBIAL LACTASE (EC 3.2.1.23) ACTIVITY IN YOGURT ON THE INTESTINAL-ABSORPTION OF LACTOSE - AN INVIVO STUDY IN LACTASE-DEFICIENT HUMANS [J].

MARTEAU, P ;

FLOURIE, B ;

POCHART, P ;

CHASTANG, C ;

DESJEUX, JF ;

RAMBAUD, JC .

BRITISH JOURNAL OF NUTRITION, 1990, 64 (01) :71-79