IbMADS4 regulates the vegetative shoot development in transgenic chrysanthemum (Dendrathema grandiflora (Ramat.) Kitamura)

被引:16
作者
Aswath, CR
Mo, SY
Kim, SH
Kim, DH
机构
[1] Univ Nacl Autonoma Mexico, Inst Ecol, Dept Ecol Evolut, Mexico City 04510, DF, Mexico
[2] Indian Inst Hort Res, Bangalore 560089, Karnataka, India
[3] Konkuk Univ, Dept Hort, Genet Engn Lab, Seoul, South Korea
关键词
IbMADS4; shoot development; chrysanthemum; transformation;
D O I
10.1016/j.plantsci.2003.11.030
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
14 A new MADS-box gene, IbMADS4, had been cloned in Ipomoea batatas (L.) Lam. that is expressed in all vegetative tissues of the plant, mainly in the root tissues, but not in flower organs. Sequence similarity besides the spatial and temporal expression patterns allows defining a novel MADS-box subfamily comprising STMADS16 and the other MADS-box genes expressed specifically in vegetative tissues. Expression of the IbMADS4 sense cDNA under the control of the 35S cauliflower mosaic virus promoter modifies dramatic changes in the phenotype, such as axillary shoot formation in chrysanthemum. Leaf and axillary shoot number of sense transgenic plants showed a three- and four-fold increase in shoot regeneration compared with the wild-type, respectively. The results presented here are aimed at understanding the molecular mechanisms involved in shoot morphogenesis. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:847 / 854
页数:8
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