Mechanism of serum resistance among Brucella abortus isolates

被引：30

作者：

Eisenschenk, FC ^{[1
]}

Houle, JJ ^{[1
]}

Hoffmann, EM ^{[1
]}

机构：

[1] Univ Florida, Inst Food & Agr Sci, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

来源：

VETERINARY MICROBIOLOGY | 1999年 / 68卷 / 3-4期

关键词：

Brucella abortus; complement; cattle-bacteria; serum resistance; O-antigen;

D O I：

10.1016/S0378-1135(99)00075-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

It was shown in this study that complement-resistant Brucella abortus used were unable to activate complement in the absence of specific antibody. Complement-resistant isolates possessed O-antigen, but complement-sensitive organisms used are O-antigen deficient. Since B. abortus LPS does not activate the alternative pathway of complement, we concluded that activation of bovine complement must be due to some other mechanism. In this study, it was shown that bovine C1 binds to the outer membrane proteins of B. abortus. Isolated outer membrane proteins of both smooth (O-antigen positive) and rough (O-antigen negative) B. abortus used bind to C1q. However, only rough isolates were killed by complement. All of the O-antigen positive B. abortus isolates were complement-resistant. We propose that O-antigen shields outer membrane proteins and blocks C1q binding. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：235 / 244

页数：10

共 23 条

[1]

Alberti S, 1996, INFECT IMMUN, V64, P4719

[2] Transposon-derived Brucella abortus rough mutants are attenuated and exhibit reduced intracellular survival [J].

Allen, CA ;

Adams, LG ;

Ficht, TA .

INFECTION AND IMMUNITY, 1998, 66 (03) :1008-1016

[3]

BETZ SJ, 1981, J IMMUNOL, V127, P1748

[4] A RAPID AND SENSITIVE METHOD FOR DETECTION AND QUANTIFICATION OF CALCINEURIN AND CALMODULIN-BINDING PROTEINS USING BIOTINYLATED CALMODULIN [J].

BILLINGSLEY, ML ;

PENNYPACKER, KR ;

HOOVER, CG ;

BRIGATI, DJ ;

KINCAID, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (22) :7585-7589

[5] PURIFICATION AND CHARACTERIZATION OF SUBCOMPONENT C1Q OF THE 1ST COMPONENT OF BOVINE COMPLEMENT [J].

CAMPBELL, RD ;

BOOTH, NA ;

FOTHERGILL, JE .

BIOCHEMICAL JOURNAL, 1979, 177 (02) :531-540

[6] ANTIBODY-INDEPENDENT BINDING OF THE 1ST COMPONENT OF COMPLEMENT (C1) AND ITS SUBCOMPONENT C1Q TO THE S-FORM AND R-FORM OF SALMONELLA-MINNESOTA [J].

CLAS, F ;

LOOS, M .

INFECTION AND IMMUNITY, 1981, 31 (03) :1138-1144

[7] KILLING OF BRUCELLA-ABORTUS BY BOVINE SERUM [J].

CORBEIL, LB ;

BLAU, K ;

INZANA, TJ ;

NIELSEN, KH ;

JACOBSON, RH ;

CORBEIL, RR ;

WINTER, AJ .

INFECTION AND IMMUNITY, 1988, 56 (12) :3251-3261

[8]

EISENSCHENK FC, 1995, AM J VET RES, V56, P1592

[9] INHIBITION OF COMPLEMENT BY A SUBSTANCE ISOLATED FROM HUMAN ERYTHROCYTES .I. EXTRACTION FROM HUMAN ERYTHROCYTE STROMATA [J].

HOFFMANN, EM .

IMMUNOCHEMISTRY, 1969, 6 (03) :391-&

[10] CONTRADICTORY ROLES FOR ANTIBODY AND COMPLEMENT IN THE INTERACTION OF BRUCELLA-ABORTUS WITH ITS HOST [J].

HOFFMANN, EM ;

HOULE, JJ .

CRITICAL REVIEWS IN MICROBIOLOGY, 1995, 21 (03) :153-163

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