Mechanism of serum resistance among Brucella abortus isolates

被引：30

作者：

Eisenschenk, FC ^{[1
]}

Houle, JJ ^{[1
]}

Hoffmann, EM ^{[1
]}

机构：

[1] Univ Florida, Inst Food & Agr Sci, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

来源：

VETERINARY MICROBIOLOGY | 1999年 / 68卷 / 3-4期

关键词：

Brucella abortus; complement; cattle-bacteria; serum resistance; O-antigen;

D O I：

10.1016/S0378-1135(99)00075-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

It was shown in this study that complement-resistant Brucella abortus used were unable to activate complement in the absence of specific antibody. Complement-resistant isolates possessed O-antigen, but complement-sensitive organisms used are O-antigen deficient. Since B. abortus LPS does not activate the alternative pathway of complement, we concluded that activation of bovine complement must be due to some other mechanism. In this study, it was shown that bovine C1 binds to the outer membrane proteins of B. abortus. Isolated outer membrane proteins of both smooth (O-antigen positive) and rough (O-antigen negative) B. abortus used bind to C1q. However, only rough isolates were killed by complement. All of the O-antigen positive B. abortus isolates were complement-resistant. We propose that O-antigen shields outer membrane proteins and blocks C1q binding. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：235 / 244

页数：10

共 23 条

[11] FAILURE OF BRUCELLA-ABORTUS LIPOPOLYSACCHARIDE (LPS) TO ACTIVATE THE ALTERNATIVE PATHWAY OF COMPLEMENT [J].

HOFFMANN, EM ;

HOULE, JJ .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1983, 5 (01) :65-76

[12] THE USE OF A PASSIVE HEMOLYSIS SYSTEM TO EVALUATE THE COMPLEMENT ACTIVITIES OF 6 MAMMALIAN-SPECIES [J].

HOULE, JJ ;

HOFFMANN, EM .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1987, 16 (3-4) :259-270

[13]

JOINER KA, 1986, J IMMUNOL, V136, P710

[14] STUDIES ON THE MECHANISM OF BACTERIAL-RESISTANCE TO COMPLEMENT-MEDIATED KILLING .2. C8 AND C9 RELEASE C5B67 FROM THE SURFACE OF SALMONELLA-MINNESOTA S218 BECAUSE THE TERMINAL COMPLEX DOES NOT INSERT INTO THE BACTERIAL OUTER-MEMBRANE [J].

JOINER, KA ;

HAMMER, CH ;

BROWN, EJ ;

FRANK, MM .

JOURNAL OF EXPERIMENTAL MEDICINE, 1982, 155 (03) :809-819

[15] STUDIES ON THE MECHANISM OF BACTERIAL-RESISTANCE TO COMPLEMENT-MEDIATED KILLING .1. TERMINAL COMPLEMENT COMPONENTS ARE DEPOSITED AND RELEASED FROM SALMONELLA-MINNESOTA S218 WITHOUT CAUSING BACTERIAL DEATH [J].

JOINER, KA ;

HAMMER, CH ;

BROWN, EJ ;

COLE, RJ ;

FRANK, MM .

JOURNAL OF EXPERIMENTAL MEDICINE, 1982, 155 (03) :797-808

[16] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[17] ANTIBODY-INDEPENDENT INTERACTION OF 1ST COMPONENT OF COMPLEMENT WITH GRAM-NEGATIVE BACTERIA [J].

LOOS, M ;

WELLEK, B ;

THESEN, R ;

OPFERKUCH, W .

INFECTION AND IMMUNITY, 1978, 22 (01) :5-9

[18]

Mayer MM., 1967, EXPT IMMUNOCHEMISTRY, P133

[19] INDIRECT HEMOLYSIS TEST (IHLT) FOR BOVINE BRUCELLOSIS [J].

PLACKETT, P ;

COTTEW, GS ;

BEST, SJ .

AUSTRALIAN VETERINARY JOURNAL, 1976, 52 (03) :136-140

[20] SDS-SOLUBLE AND PEPTIDOGLYCAN-BOUND PROTEINS IN THE OUTER-MEMBRANE PEPTIDOGLYCAN COMPLEX OF BRUCELLA-ABORTUS [J].

SOWA, BA ;

KELLY, KA ;

FICHT, TA ;

FREY, M ;

ADAMS, LG .

VETERINARY MICROBIOLOGY, 1991, 27 (3-4) :351-369

← 1 2 3 →