The Mushroom Body of Adult Drosophila Characterized by GAL4 Drivers

被引:276
作者
Aso, Yoshinori [1 ,2 ]
Grubel, Kornelia [2 ]
Busch, Sebastian [1 ,2 ]
Friedrich, Anja B. [1 ]
Siwanowicz, Igor [1 ]
Tanimoto, Hiromu [1 ,2 ]
机构
[1] Max Planck Inst Neurobiol, D-82152 Martinsried, Germany
[2] Univ Wurzburg, Dept Genet & Neurobiol, Wurzburg, Germany
关键词
insect brain; morphology; GAL4; UAS system; expression database; Kenyon cells; LONG-TERM-MEMORY; CELL-AUTONOMOUS REQUIREMENT; GREEN FLUORESCENT PROTEIN; CENTRAL-NERVOUS-SYSTEM; FUNCTIONAL DISSECTION; GENE-EXPRESSION; NEUROBLAST PROLIFERATION; SYNAPTIC-TRANSMISSION; DENDRITIC ELABORATION; TRANSGENE EXPRESSION;
D O I
10.1080/01677060802471718
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The mushroom body is required for a variety of behaviors of Drosophila melanogaster. Different types of intrinsic and extrinsic mushroom body neurons might underlie its functional diversity. There have been many GAL4 driver lines identified that prominently label the mushroom body intrinsic neurons, which are known as Kenyon cells. Under one constant experimental condition, we analyzed and compared the the expression patterns of 25 GAL4 drivers labeling the mushroom body. As an internet resource, we established a digital catalog indexing representative confocal data of them. Further more, we counted the number of GAL4-positive Kenyon cells in each line. We found that approximately 2,000 Kenyon cells can be genetically labeled in total. Three major Kenyon cell subtypes, the , '/', and / neurons, respectively, contribute to 33, 18, and 49% of 2,000 Kenyon cells. Taken together, this study lays groundwork for functional dissection of the mushroom body.
引用
收藏
页码:156 / U29
页数:24
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