Constitutive and inducible nitric oxide synthase activities after spinal cord contusion in rats

被引：60

作者：

Díaz-Ruiz, A

Ibarra, A

Pérez-Severiano, F

Guízar-Sahagún, G

Grijalva, I

Ríos, C

机构：

[1] Inst Nacl Neurol & Neurocirugia Manuel Velasco Su, Dept Neuroquim, Mexico City, DF, Mexico

[2] IMSS, CMN Siglo XXI, HE, Unidad Invest Med Enfermedades Neurol, Mexico City, DF, Mexico

[3] Proyecto Camina AC, Lab Neuroinmunol, Mexico City, DF, Mexico

来源：

NEUROSCIENCE LETTERS | 2002年 / 319卷 / 03期

关键词：

nitric oxide; synthase; inducible; constitutive; spinal cord injury;

D O I：

10.1016/S0304-3940(01)02540-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Nitric oxide (NO) plays a role in the secondary damage after spinal cord (SC) injury. NO is produced by the activity of two classes of enzymes: calcium-dependent constitutive nitric oxide synthase (NOS) and calcium-independent inducible NOS. To determine the time course of both NOS activities after SC injury, 50 Wistar rats were submitted to severe SC contusion. NOS activities were assayed at the site of SC injury at several times after lesion. Results showed a significant increase of 138 and 96% in the constitutive NOS activity at 4. and 8 h after the lesion, respectively, as compared to sham-operated rats. iNOS activity was increased 72 h after lesion by 103% (P < 0.05). In conclusion, both isoforms of NOS increase their activity at different time periods after SC injury. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：129 / 132

页数：4

共 20 条

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