Metabolism of Reactive Nitrogen Species in Pea Plants Under Abiotic Stress Conditions

被引:205
作者
Corpas, Francisco J. [1 ]
Chaki, Mounira [2 ]
Fernandez-Ocana, Ana [2 ]
Valderrama, Raquel [2 ]
Palma, Jose M. [1 ]
Carreras, Alfonso [2 ]
Begara-Morales, Juan C. [2 ]
Airaki, Morad [1 ]
del Rio, Luis A. [1 ]
Barroso, Juan B. [2 ]
机构
[1] CSIC, Estac Expt Zaidin, Dept Bioquim Biol Celular & Mol Plantas, E-18080 Granada, Spain
[2] Univ Jaen, Area Bioquim & Biol Mol, Unidad Asociada CSIC, Grp Senalizac Mol & Syst Antioxidantes Plantas, E-23071 Jaen, Spain
关键词
D O I
10.1093/pcp/pcn144
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Nitric oxide (NO) is a key signaling molecule in different physiological processes of animals and plants. However, little is known about the metabolism of endogenous NO and other reactive nitrogen species (RNS) in plants under abiotic stress conditions. Using pea plants exposed to six different abiotic stress conditions (high light intensity, low and high temperature, continuous light, continuous dark and mechanical wounding), several key components of the metabolism of RNS including the content of NO, S-nitrosothiols (RSNOs) and nitrite plus nitrate, the enzyme activities of arginine-dependent nitric oxide synthase (NOS) and S-nitrosogluthathione reductase (GSNOR), and the profile of protein tyrosine nitration (NO2-Tyr) were analyzed in leaves. Low temperature was the stress that produced the highest increase of NOS and GSNOR activities, and this was accompanied by an increase in the content of total NO and S-nitrosothiols, and an intensification of the immunoreactivity with an antibody against NO2-Tyr. Mechanical wounding, high temperature and light also had a clear activating effect on the different indicators of RNS metabolism in pea plants. However, the total content of nitrite and nitrate in leaves was not affected by any of these stresses. Considering that protein tyrosine nitration is a potential marker of nitrosative stress, the results obtained suggest that low and high temperature, continuous light and high light intensity are abiotic stress conditions that can induce nitrosative stress in pea plants.
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页码:1711 / 1722
页数:12
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