Enzymatic syntheses of GlcNAc beta 1-2Man and Gal beta 1-4GlcNAc beta 1-2Man as components of complex type sugar chains

被引：16

作者：

Fujimoto, H

Isomura, M

Miyazaki, T

Matsuo, I

Walton, R

Sakakibara, T

Ajisaka, K

机构：

[1] MEIJI INST HLTH SCI,ODAWARA 250,JAPAN

[2] YOKOHAMA CITY UNIV,DEPT CHEM,FAC SCI,YOKOHAMA,KANAGAWA 236,JAPAN

来源：

GLYCOCONJUGATE JOURNAL | 1997年 / 14卷 / 01期

关键词：

GlcNAc beta 1-2Man; Gal beta 1-4GlcNAc beta 1-2Man; Gal beta 1-4GlcNAc beta 1-6Man; components of complex type sugar chains;

D O I：

10.1023/A:1018565032297

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

GlcNAc beta 1-2Man and GlcNAc beta 1-6Man were synthesized using the reverse hydrolysis activity of beta-N-acetylglucosaminidase from both jack beans and Bacillus circulans. In turn, Gal beta 1-4GlcNAc beta 1-2Man and Gal beta 1-4GlcNAc beta 1-6Man were synthesized regioselectively using the transglycosylation activity of beta-galactosidase from Diplococcus pneumoniae and B. circulans, respectively. These di- and trisaccharides are important components of complex type sugar chains and will be used as intermediates in our synthetic studies.

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页码：75 / 80

页数：6

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