How many dendritic cells are required to initiate a T-cell response?

被引：65

作者：

Celli, Susanna ^{[2
]}

Day, Mark ^{[3
]}

Mueller, Andreas J. ^{[2
]}

Molina-Paris, Carmen ^{[3
]}

Lythe, Grant ^{[3
]}

Bousso, Philippe ^{[1
,2
]}

机构：

[1] Inst Pasteur, Dept Immunol, Dynam Immune Responses Unit, F-75015 Paris, France

[2] Inserm, U668, Paris, France

[3] Univ Leeds, Dept Appl Math, Leeds LS2 9JT, W Yorkshire, England

来源：

BLOOD | 2012年 / 120卷 / 19期

基金：

英国生物技术与生命科学研究理事会; 欧洲研究理事会;

关键词：

IN-VIVO; LYMPH-NODE; ANTIGEN; ACTIVATION; MOTILITY; REVEALS;

D O I：

10.1182/blood-2012-01-408260

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

T-cell activation in lymph nodes relies on encounters with antigen (Ag)-bearing dendritic cells (DCs) but the number of DCs required to initiate an immune response is unknown. Here we have used a combination of flow cytometry, 2-photon imaging, and computational modeling to quantify the probability of T cell-DC encounters. We calculated that the chance for a T cell residing 24 hours in a murine popliteal lymph nodes to interact with a DC was 8%, 58%, and 99% in the presence of 10, 100, and 1000 Ag-bearing DCs, respectively. Our results reveal the existence of a threshold in DC numbers below which T-cell responses fail to be elicited for probabilistic reasons. In mice and probably humans, we estimate that a minimum of 85 DCs are required to initiate a T-cell response when starting from precursor frequency of 10(-6). Our results have implications for the rational design of DC-based vaccines. (Blood. 2012;120(19):3945-3948)

引用

页码：3945 / 3948

页数：4

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