On the signal response of various pesticides in electrospray and atmospheric pressure chemical ionization depending on the flow-rate of eluent applied in liquid chromatography-tandem mass spectrometry

被引:45
作者
Asperger, A [1 ]
Efer, R [1 ]
Koal, T [1 ]
Engewald, W [1 ]
机构
[1] Univ Leipzig, Inst Analyt Chem, D-04103 Leipzig, Germany
关键词
flow-rate; signal response; atmospheric pressure ionization; mass spectrometry; electrospray ionization; pesticides;
D O I
10.1016/S0021-9673(01)01296-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The API-MS signal response of several pesticides (atrazine, simazine, isoproturon, diuron, chlorfenvinphos, chlorpyrifos, alachlor, trifluralin) depending on the flow-rate of eluent entering the MS interface was investigated. The investigations were based on API-MS-MS analyses of standard pesticide mixtures in the flow injection mode (FIA) at systematically varied eluent flow-rates using both an ESI interface (Turboionspray) and a heated nebulizer type APCI source. In the result, the individual compounds included in this study showed significant differences in their signal response behaviour depending on the flow-rate of eluent applied. The most hydrophobic compounds among the investigated pesticides (chlorpyrifos and trifluralin) showed drastic losses of sensitivity with increasing eluent flow-rate in both ESI and APCI, while more hydrophilic compounds like atrazine, simazine and isoproturon showed the expected signal response (concentration-sensitive in ESI, mass-flow-sensitive in APCI) at least within a certain range of flow-rates (200-600 mul/min in ESI, 200-2000 mul/min in APCI). These findings lead to the conclusion that application of a programmed HPLC eluent flow-rate may be advantageous to achieve maximum sensitivity of API-MS detection for all pesticides of interest. This is exemplified by the implementation of a flow gradient into an online SPE-HPLC-APCI-MS/MS method for improved analysis of pesticides in drinking water. (C) 2001 Elsevier Science B.V. All rights reserved.
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页码:65 / 72
页数:8
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