Persistence of cadmium-induced adaptive response to genotoxicity of maleic hydrazide and methyl mercuric chloride in root meristem cells of Allium cepa L: Differential inhibition by cycloheximide and buthionine sulfoximine

被引:14
作者
Panda, KK [1 ]
Patra, J [1 ]
Panda, BB [1 ]
机构
[1] BERHAMPUR UNIV,DEPT BOT,GENET TOXICOL LAB,BERHAMPUR 760007,ORISSA,INDIA
关键词
metallo-adaptive response; phytochelatin; genotoxicity; maleic hydrazide; methylmercuric chloride; allium MNC assay;
D O I
10.1016/S1383-5718(96)00131-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
With an objective to determine the period of persistence of the metal-induced adaptive response to chemical mutagens and he;ivy metals, growing root meristems of Allium cepa were conditioned by cadmium sulfate (CdSO4), 3 x 10(-7) and 4 x 10(-6) M for 1 h and subsequently challenged by maleic hydrazide (MH), 5 x 10(-3) M or methyl mercuric chloride (MMCl), 1.26 x 10(-6) M for 3 h at different time intervals ranging from a few minutes to several hours following the conditioning dose. Root meristems, fixed at regular intervals during recovery from 6 to 48 h, were cytologically analysed for cells with micronuclei (MNC). The adaptive responses to MH and MMCl were observed as early as 5 min after the Cd-conditioning that persisted for at least 48 h. Metabolic inhibitors, cycloheximide (CH), 10(-7) M and buthionine sulfoximine (BSO), 10(-4) M administered either prior to or simultaneous with Cd-conditioning effectively prevented the adaptive response to MH. Whereas BSO, an inhibitor of phytochelatin synthesis, prevented the adaptive responses from 15 min to 8 h after the conditioning dose, CH an inhibitor of cytoplasmic protein synthesis prevented the same from 6 to 48 h. The findings underscored the differential roles of phytochelatins and proteins underlying the foregone metallo-adaptive response.
引用
收藏
页码:129 / 139
页数:11
相关论文
共 38 条
[31]   RESIDUAL MERCURY IN SEED OF BARLEY (HORDEUM-VULGARE L) CONFERS GENOTOXIC ADAPTATION TO ETHYL METHANESULFONATE, MALEIC HYDRAZIDE, METHYL MERCURIC-CHLORIDE AND MERCURY-CONTAMINATED SOIL [J].
SUBHADRA, AV ;
PANDA, KK ;
PANDA, BB .
MUTATION RESEARCH, 1993, 300 (3-4) :141-149
[32]   METAL-INDUCED GENOTOXIC ADAPTATION IN BARLEY (HORDEUM-VULGARE L) TO MALEIC HYDRAZIDE AND METHYL MERCURIC-CHLORIDE [J].
SUBHADRA, AV ;
PANDA, BB .
MUTATION RESEARCH, 1994, 321 (1-2) :93-102
[33]  
SUBHADRA AV, 1992, BIOL ZENT BL, V3, P120
[34]   POSSIBLE ROLE FOR METALLOTHIONEIN IN PROTECTION AGAINST RADIATION-INDUCED OXIDATIVE STRESS - KINETICS AND MECHANISM OF ITS REACTION WITH SUPEROXIDE AND HYDROXYL RADICALS [J].
THORNALLEY, PJ ;
VASAK, M .
BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 827 (01) :36-44
[35]   CHANGES IN GLUTATHIONE AND PHYTOCHELATINS IN ROOTS OF MAIZE SEEDLINGS EXPOSED TO CADMIUM [J].
TUKENDORF, A ;
RAUSER, WE .
PLANT SCIENCE, 1990, 70 (02) :155-166
[36]  
Vangronsveld Jaco, 1994, P149, DOI 10.1002/9783527615919.ch6
[37]   INHIBITION OF THE ADAPTIVE RESPONSE OF HUMAN-LYMPHOCYTES TO VERY LOW-DOSES OF IONIZING-RADIATION BY THE PROTEIN-SYNTHESIS INHIBITOR CYCLOHEXIMIDE [J].
YOUNGBLOM, JH ;
WIENCKE, JK ;
WOLFF, S .
MUTATION RESEARCH, 1989, 227 (04) :257-261
[38]  
ZHOW J, 1994, PLANT CELL, V6, P875