Complementary effects of bifidogenic growth stimulators and ammonium sulfate in natural rubber serum powder on Bifidobacterium bifidum

被引：8

作者：

Etoh, S ^{[1
]}

Sonomoto, K ^{[1
]}

Ishizaki, A ^{[1
]}

机构：

[1] Kyushu Univ, Fac Agr, Dept Food Sci & Technol, Lab Microbial Technol,Higashi Ku, Fukuoka 8128581, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 04期

关键词：

natural rubber serum powder; Bifidobacterium; bifidogenic growth stimulator; ammonium sulfate;

D O I：

10.1271/bbb.63.627

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Natural rubber serum powder, rich in crude protein and carbohydrates, had a strong growth-stimulating activity for Bifidobacterium bifidum JCM 1254, which was unable to grow in a fully synthetic medium, B-12 assay medium. Natural rubber serum powder was fractionated by ultrafiltration (molecular weight cutoff 1000). The active ultrafiltrate was further concentrated and desalted with an adsorptive microconcentrator, which adsorbs virtually all amino acids and peptides. Through this purification step, it was found that the adsorbed fraction obtained did not stimulate growth independently but acted complementarily with a small amount of ammonium sulfate. The adsorbed fraction was subsequently analyzed on reversed-phase high pressure liquid chromatography, and the activities of the eluates were measured on B-12 assay medium with ammonium sulfate. Consequently, it was proved that several peptidic ingredients in the adsorbed fraction increased the growth of B. bifidum.

引用

页码：627 / 631

页数：5

共 31 条

[1] IDENTIFICATION OF A GROWTH STIMULANT FOR LACTOBACILLUS-SANFRANCISCO [J].