DEVELOPMENT OF CORONARY VESSELS

被引：9

作者：

Dong, Xiu Rong ^{[1
,2
]}

Maguire, Colin T. ^{[1
,4
]}

Wu, San-Pin ^{[5
]}

Majesky, Mark W. ^{[1
,2
,3
,4
]}

机构：

[1] Univ N Carolina, Carolina Cardiovasc Biol Ctr, Chapel Hill, NC 27515 USA

[2] Univ N Carolina, Dept Med, Chapel Hill, NC USA

[3] Univ N Carolina, Dept Genet, Chapel Hill, NC USA

[4] Baylor Coll Med, Cardiovasc Sci Grad Program, Houston, TX 77030 USA

[5] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA

来源：

ANGIOGENESIS: IN VIVO SYSTEMS, PT B | 2008年 / 445卷

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0076-6879(08)03009-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

This chapter summarizes experimental techniques used to study coronary vessel development from its origins in the proepicardium (PE) to the final assembled network of arteries, veins, and capillaries present in the mature heart. Methods are described for microdissection and culture of the PE and embryonic epicardial cells, isolation of total RNA from single PE primordia and analysis by RT-PCR, imaging of the epicardium and coronary vessels by whole-mount confocal microscopy and by scanning electron microscopy, and the preparation of coronary vascular corrosion casts to visualize the entire coronary artery network structure. These techniques form the basic tools to study the cellular and molecular pathways that guide development and remodeling of coronary vessels.

引用

页码：209 / +

页数：23

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