Two highly related homeodomain proteins, Nkx5-1 and Nkx5-2, display different DNA binding specificities

被引：10

作者：

Mennerich, D ^{[1
]}

Hoffmann, S ^{[1
]}

Hadrys, T ^{[1
]}

Arnold, HH ^{[1
]}

Bober, E ^{[1
]}

机构：

[1] Tech Univ Braunschweig, Dept Cell & Mol Biol, D-38106 Braunschweig, Germany

来源：

BIOLOGICAL CHEMISTRY | 1999年 / 380卷 / 09期

关键词：

binding site selection; homeodomain; Nkx5; genes; transcription factors;

D O I：

10.1515/BC.1999.130

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mouse Nkx5-1 and Nkx5-2 genes are related to NK genes in Drosophila and encode proteins with very similar homeodomains. In higher vertebrates Nkx5 genes are specifically expressed in the inner ear. Inactivation of the mouse Nkx5-1 gene by homologous recombination revealed a critical role for the formation of vestibular inner ear structures. Here, we investigated biochemical properties of the proteins encoded by the Nkx5 genes. A similar consensus binding sequence was isolated for both Nkx5 proteins using binding site selection. This sequence is related to target sequences previously identified for other Nkx proteins and contains the conserved homeodomain binding core TAAT. An additional, novel and unrelated high affinity binding sequence could be identified for the Nkx5-2 protein.

引用

页码：1041 / 1048

页数：8

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