Molecular characterization of cytokinin-responsive histidine kinases in maize. Differential ligand preferences and response to cis-zeatin

被引：160

作者：

Yonekura-Sakakibara, K ^{[1
]}

Kojima, M ^{[1
]}

Yamaya, T ^{[1
]}

Sakakibara, H ^{[1
]}

机构：

[1] RIKEN, Inst Phys & Chem Res, Plant Sci Ctr, Yokohama, Kanagawa 2300045, Japan

来源：

PLANT PHYSIOLOGY | 2004年 / 134卷 / 04期

关键词：

D O I：

10.1104/pp.103.037176

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Genes for cytokinin-responsive His-protein kinases (ZmHK1, ZmHK2, and ZmHK3a) were isolated from maize (Zea mays). Heterologous expression of each of the ZmHKs in Escherichia coli having the DeltarcsC and cps::lacZ genetic background conferred cytokinin-inducibility of lacZ expression on the bacteria. In the recombinant E. coli system, ZmHK1 and ZmHK3a were more sensitive to free-base cytokinins than to the corresponding nucleosides; isopentenyladenine was most effective for ZmHK1, while ZmHK2 tended to be most sensitive to trans-zeatin and the riboside. In contrast to a known cytokinin receptor of Arabidopsis (AHK4/CRE1/WOL), all ZmHKs responded to cis-zeatin (cZ), which generally is believed to be inactive or only weakly active. In cultured maize cells, expression of ZmRR1, a cytokinin-inducible response regulator, was induced by cZ as well as by trans-zeatin. These results strongly suggest that maize cytokinin receptors differ in ligand preference, and that cZ is an active cytokinin at least in maize.

引用

页码：1654 / 1661

页数：8

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