A metacaspase of Trypanosoma brucei causes loss of respiration competence and clonal death in the yeast Saccharomyces cerevisiae

被引：113

作者：

Szallies, A

Kubata, BK

Duszenko, M

机构：

[1] Univ Tubingen, Verfugungsgebaude, D-72076 Tubingen, Germany

[2] Univ Tubingen, Inst Physiol Chem, D-72076 Tubingen, Germany

[3] Osaka Biosci Inst, Dept Mol Behav Biol, Osaka 5650874, Japan

来源：

FEBS LETTERS | 2002年 / 517卷 / 1-3期

关键词：

metacaspase; cysteine protease; WW domain; mitochondrion; Saccharomyces cerevisiae; Trypanosoma brucei;

D O I：

10.1016/S0014-5793(02)02608-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metacaspases constitute a new group of cysteine proteases homologous to caspases. Heterologous expression of Trypanosoma brucei metacaspase TbMCA4 in the budding yeast Saccharomyces cerevisiae resulted in growth inhibition, mitochondrial dysfunction and clonal death. The metacaspase orthologue of yeast, ScMCA1 (YOR197w), exhibited genetic interaction with WWM1 (YFL010c), which encodes a small WW domain protein. WWM1 overexpression resulted in growth arrest and clonal death, which was suppressed by concomitant overexpression of ScMCA1. GFP-fusion reporters of WWM1, ScMCA1 and TbMCA4 localized to the nucleus. Taken together, we suggest that metacaspases may play a role in nuclear function controlling cellular proliferation coupled to mitochondrial biogenesis. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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页码：144 / 150

页数：7

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