Protective effect of sodium selenite on ofloxacin-induced loss of chloroplast DNA in Euglena gracilis

被引：15

作者：

Krizkova, L

Horniak, L

Slavikova, S

Ebringer, L

机构：

[1] Inst. Molec. and Subcellular Biol., Faculty of Science, Comenius University

来源：

FOLIA MICROBIOLOGICA | 1996年 / 41卷 / 04期

关键词：

D O I：

10.1007/BF02814709

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Some xenobiotics induce permanent loss of chloroplasts in Euglena gracilis which results in the growth of white mutant colonies (bleaching effect). The effect of ofloxacin and sodium selenite on the organization of chloroplast DNA of E. gracilis was studied by fluorescent microscopy. In the presence of ofloxacin DNA-specific staining with DAPI revealed a decrease in chloroplast DNA content and in the number of nucleoids per chloroplast. This was accompanied with a decrease of chloroplast number per cell and with the loss of stigma. Spectrophotometry of chlorophyll extract revealed changes in the ratio of chi a :chi b. The presence of sodium selenite protected chloroplast DNA against the inhibitory effect of ofloxacin.

引用

页码：329 / 332

页数：4

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