Sensitive assay for determining plasma tenofovir concentrations by LC/MS/MS

被引：85

作者：

Delahunty, T ^{[1
]}

Bushman, L ^{[1
]}

Fletcher, CV ^{[1
]}

机构：

[1] Univ Colorado, Antiviral Pharmacol Lab, Dept Clin Pharm, Hlth Sci Ctr,Sch Pharm, Denver, CO 80262 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 830卷 / 01期

关键词：

tenofovir (TNF); adefovir (PMEA); LC/MS/MS; plasma;

D O I：

10.1016/j.jchromb.2005.10.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An LC/MS/MS assay for the determination of tenofovir (TNF) was developed and validated for use with the EDTA anticoagulated human plasma matrix. Heparin-treated plasma and serum matrices were also validated. After addition of adefovir as an internal standard, trifluoroacetic acid was used to produce a protein-free extract. Chromatographic separation was achieved with a Polar-RP Synergi, 2.0 mm x 150 mm, reversed-phase analytical column. The mobile phase was 3% acetonitrile/1% acetic acid, aq. Detection of TNF and the internal standard was achieved by ESI MS/MS in the positive ion mode using 288/176 and 274/162 transitions, respectively. The method was linear from 10 to 750 ng/ml with a minimum quantifiable limit of 10 ng/ml when 250 mu l aliquots were analyzed. The usefulness of this LC/MS/MS method to routinely monitor plasma concentrations of TNF was demonstrated alone with its ability to assist in the performance of pharmacokinetic studies. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：6 / 12

页数：7