Analysis of expression of GroEL (Hsp60) of Clostridium difficile in response to stress

被引：49

作者：

Hennequin, C ^{[1
]}

Collignon, A ^{[1
]}

Karjalainen, T ^{[1
]}

机构：

[1] Univ Paris Sud, Fac Pharm, Dept Microbiol, F-92296 Chatenay Malabry, France

来源：

MICROBIAL PATHOGENESIS | 2001年 / 31卷 / 05期

关键词：

Clostridium difficile; stress response; GroEL; hsp60; RT-PCR; immunoblot;

D O I：

10.1006/mpat.2001.0468

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Our laboratory has previously shown that adherence of Clostridium difficile to tissue culture cells is augmented by various stresses and that GroEL, a heat shock protein, serves an adhesive function in this bacterium. In this communication, RT-PCR, SIDS-PAGE and immunoblotting were used to study the stress response in C. difficile following heat, acid or osmotic shock, iron deprivation or presence of a subinhibitory concentration of ampicillin in the culture medium. All these stresses increased transcription of groEL and production of GroEL to various degrees. Furthermore, the protein was found in membrane fractions and in the extracellular space after heat stress. (C) 2001 Academic Press.

引用

页码：255 / 260

页数：6

共 17 条

[11]

SHINNICK TM, 1991, CURR TOP MICROBIOL, V167, P145

[12] Molecular characterization of fliD gene encoding flagellar cap and its expression among Clostridium difficile isolates from different serogroups [J].

Tasteyre, A ;

Karjalainen, T ;

Avesani, V ;

Delmée, M ;

Collignon, A ;

Bourlioux, P ;

Barc, MC .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (03) :1178-1183

[13] ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].

TOWBIN, H ;

STAEHELIN, T ;

GORDON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354

[14]

Waligora AJ, 1999, APPL ENVIRON MICROB, V65, P4234

[15] Characterization of a cell surface protein of Clostridium difficile with adhesive properties [J].

Waligora, AJ ;

Hennequin, C ;

Mullany, P ;

Bourlioux, P ;

Collignon, A ;

Karjalainen, T .

INFECTION AND IMMUNITY, 2001, 69 (04) :2144-2153

[16]

WEXLER H, 1984, REV INFECT DIS, V6, pS229

[17] Role of heat shock proteins in protection from and pathogenesis of infectious diseases [J].

Zügel, U ;

Kaufmann, SHE .

CLINICAL MICROBIOLOGY REVIEWS, 1999, 12 (01) :19-+

← 1 2 →