Value of the sperm chromatin dispersion test in predicting pregnancy outcome in intrauterine insemination:: a blind prospective study

被引：79

作者：

Muriel, L

Meseguer, M

Fernández, JL

Alvarez, J

Remohí, J

Pellicer, A

Garrido, N

机构：

[1] Inst Valenciano Infertil, Androl Lab & Semen Bank, Valencia 46015, Spain

[2] Inst Univ IVI, Valencia, Spain

[3] FIVI Fdn IVI, Valencia, Spain

[4] Ctr Infertilidad Masculina ANDROGEN, La Coruna, Spain

[5] Harvard Univ, Sch Med, Boston, MA 02115 USA

来源：

HUMAN REPRODUCTION | 2006年 / 21卷 / 03期

关键词：

DNA; intrauterine insemination; sperm; sperm chromatin dispersion test;

D O I：

10.1093/humrep/dei403

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

BACKGROUND: Sperm DNA integrity has been used as a new marker of sperm quality in the prediction of pregnancy. Nevertheless, no previous study has been performed by analysing the same samples that were employed in assisted reproduction. The main objective of this work was to correlate sperm chromatin dispersion (SCD), measured by the SCD test, with semen parameters and pregnancy outcome in intrauterine insemination (IUI). METHODS: A total of 100 semen samples obtained from males of couples undergoing IUI were analysed by the SCD test before and after swim-up, and the results were correlated with semen parameters and pregnancy outcome. RESULTS: SCD was negatively correlated with sperm motility in both ejaculated and processed semen. Sperm recovered by swim-up did not show a significant improvement in DNA integrity. No correlation was found between SCD and pregnancy outcome in IUI. CONCLUSIONS: DNA dispersion, as measured by the SCD test, is not correlated with pregnancy outcome in IUI.

引用

页码：738 / 744

页数：7

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[11] Relationship among standard semen parameters, glutathione peroxidase/glutathione reductase activity, and mRNA expression and reduced glutathione content in ejaculated spermatozoa from fertile and infertile men [J].